Effect of circulating tissue factor on hypercoagulability in type 2 diabetes mellitus studied by rheometry and dielectric blood coagulometry

IF 1 4区医学 Q4 BIOPHYSICS Biorheology Pub Date : 2016-11-16 DOI:10.3233/BIR-16107

I. Uchimura, M. Kaibara, M. Nagasawa, Y. Hayashi

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引用次数: 2

Abstract

Background: Hypercoagulability in type 2 diabetes mellitus (T2DM) patients increases their risk of cardiovascular diseases. Objective: The aim of this work was to investigate the hypercoagulation mechanism in T2DM patients in terms of circulating tissue factor (TF). Methods: Whole blood coagulation tests by damped oscillation rheometry and dielectric blood coagulometry (DBCM) were performed. Results: The average coagulation time was significantly shorter for T2DM patients than for healthy controls. In vitro addition of either anti-TF or anti-activated factor VII (FVIIa) antibody to hypercoagulable blood samples prolonged coagulation times for one group of patients, while coagulation times remained short for another group. The levels of circulating TF were estimated in the former group by measuring the coagulation times for blood samples from healthy subjects with addition of various concentrations of TF and comparing them with the coagulation times for the group. The results indicated that the levels of circulating TF were on the order of subpicomolar at most. Conclusions: Circulating TF is at least partially responsible for a hypercoagulable group of T2DM patients, while an abnormality in the intrinsic coagulation pathway probably occurs in the other group.

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采用流变仪和介电凝血法研究循环组织因子对2型糖尿病患者高凝性的影响

背景:2型糖尿病(T2DM)患者的高凝血症增加了心血管疾病的风险。目的:从循环组织因子(TF)的角度探讨T2DM患者的高凝机制。方法:采用阻尼振荡流变仪和介电凝血仪进行全血凝血试验。结果:T2DM患者的平均凝血时间明显短于健康对照组。在高凝血样本中体外添加抗tf或抗活化因子VII (FVIIa)抗体可延长一组患者的凝血时间，而另一组患者的凝血时间仍短。在前一组中，通过测量健康受试者血液样本在添加不同浓度的TF后的凝血时间，并将其与对照组的凝血时间进行比较，来估计循环TF的水平。结果表明，循环TF水平最高为亚皮摩尔数量级。结论:循环TF至少是T2DM高凝组患者的部分原因，而另一组患者可能存在内在凝血途径异常。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.