Response surface methodology-aided maceration method optimization of quercetin-standardized purified extract of shallot skin (Allium cepa L var. aggregatum)

Abstract

: Shallot skin has potential as a medicinal raw material because it contains many quercetin compounds. It is necessary to conduct a study on the optimization of extraction methods and the extracts standardization. The purpose of this study is the standardization of simplicia, optimization of extraction methods and standardization of shallot skin purified extract as standardized on quercetin. This study is aimed to support quality control of simplicia and shallot skin extract as a source of quercetin. The methods of the study include the collection of raw materials, preparation of simplicia, standardization of simplicia, and optimization of extraction methods with Response Surface Methodology (RSM) using Box-Behnken Design (BBD), standardization of standardized quercetin purified extracts. Based on the results obtained, shallot skin simplicia has a moisture content of 7.98 and a total ash content of 9.91%. Based on the analysis, the optimum point of the solvent concentration factor, time, and the solvent ratio on the yield of the purified extract were respectively 60.42%, 1.29 hours, and 24.45 mL/g. The yield average obtained was 5.16 ± 0.1125 % with a content of 5.947 ppm (0.005497 mg/g).