Regulation of gene expression in Nicotiana tabacum seedlings by the MKASAA peptide through DNA methylation via the RdDM pathway

IF 1.1 Q4 BIOPHYSICS AIMS Biophysics Pub Date : 2022-01-01 DOI:10.3934/biophy.2022011
L. Fedoreyeva, Tatiana A. Dilovarova, B. Vanyushin, I. Chaban, N. Kononenko
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Abstract

DNA methylation is involved in the protection of the genome, the regulation of gene expression, splicing, and is associated with a serious reprogramming of plant development. Using fluorescence microscopy, it was shown that the MKASAA peptide penetrates through the root system of Nicotiana tabacum tobacco, mainly into the cap, meristem, and elongation zones. In the cell, the peptide is localized mainly on the nuclei. In tobacco seedlings grown in the presence of the peptide at a concentration of 10–7 M, an increase in the expression of DNA methyltransferases, especially DRM2, which methylates previously unmethylated DNA sites, is observed. In the presence of the peptide in the roots and leaves of tobacco, the level of global DNA methylation increases. An increase in DNA methylation occurs via the RdDM pathway. Presumably, the peptide binds to siRNAs, forming giant particles that remodulate chromatin and facilitate the entry of DNA methyltransferases. An increase in the level of DNA methylation is accompanied by silencing of the genes of the GRF, KNOX, and EXP families. Suppression of gene expression of these families is accompanied by significant morphological changes in tobacco seedlings. Thus, the short exogenous MKASAA peptide is involved in global morphological and genetic changes in tobacco seedlings.
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MKASAA肽通过RdDM途径DNA甲基化调控烟草幼苗基因表达
DNA甲基化涉及基因组的保护、基因表达的调控、剪接,并与植物发育的严重重编程有关。荧光显微镜显示,MKASAA肽在烟草根系中渗透,主要渗透到烟叶帽区、分生组织和伸长区。在细胞中,肽主要定位在细胞核上。在10-7 M浓度的多肽存在下生长的烟草幼苗中,观察到DNA甲基转移酶的表达增加,特别是DRM2,它将先前未甲基化的DNA位点甲基化。在烟草根和叶中存在肽时,全球DNA甲基化水平增加。DNA甲基化的增加通过RdDM途径发生。据推测,这种肽与sirna结合,形成巨大的颗粒,调节染色质,促进DNA甲基转移酶的进入。DNA甲基化水平的增加伴随着GRF、KNOX和EXP家族基因的沉默。这些家族基因表达的抑制伴随着烟草幼苗显著的形态变化。因此,外源短肽MKASAA参与了烟草幼苗的整体形态和遗传变化。
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来源期刊
AIMS Biophysics
AIMS Biophysics BIOPHYSICS-
CiteScore
2.40
自引率
20.00%
发文量
16
审稿时长
8 weeks
期刊介绍: AIMS Biophysics is an international Open Access journal devoted to publishing peer-reviewed, high quality, original papers in the field of biophysics. We publish the following article types: original research articles, reviews, editorials, letters, and conference reports. AIMS Biophysics welcomes, but not limited to, the papers from the following topics: · Structural biology · Biophysical technology · Bioenergetics · Membrane biophysics · Cellular Biophysics · Electrophysiology · Neuro-Biophysics · Biomechanics · Systems biology
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