Effect of Rs5746136 genotypes on SOD activity and biomarkers levels in breast cancer patients

IF 1.1 Q4 BIOPHYSICS AIMS Biophysics Pub Date : 2022-01-01 DOI:10.3934/biophy.2023001
H. Abdulabbas, Y. H. Al-Mawlah
{"title":"Effect of Rs5746136 genotypes on SOD activity and biomarkers levels in breast cancer patients","authors":"H. Abdulabbas, Y. H. Al-Mawlah","doi":"10.3934/biophy.2023001","DOIUrl":null,"url":null,"abstract":"Oxidative stress factors are among the most common carcinogens, Superoxide dismutase enzyme-2 (SOD2) is an endogenous antioxidant involved in the scavenging of superoxide anions. This study aimed to investigate the effect of the SOD2 gene polymorphism (rs5746136) on SOD activity and biomarker levels in breast cancer patients. This study aimed to investigate the effect of SOD2 gene (rs5746136) polymorphisms on SOD activity and biomarkers levels in breast cancer patients. The spectrophotometry methods were used to detect malondialdehyde (MDA) and Catalase (CAT), Superoxide dismutase (SOD), and Glutathione (GSH) levels, which reflect antioxidant capacity, and the genotypes of rs5746136 were detected utilize PCR and RFLP. According to the current findings, the GA genotype of the control group was the most common (70%), followed by GG and AA genotypes (26.7% and 3.3%) respectively. In the patient group, the most common genotype was GG (45.6%), followed by the GA genotype (42.8%) and then the AA genotype (11.4%) The frequency of heterozygous genotype G/A compared to the homozygous genotype (G/G) [OR = 0.3571, 95% CI = 0.1375–0.9277, P = 0.0345]. The AA genotype is significantly associated with an increased risk of developing BC [OR = 2.00, p = 0.5403, CI: 0.2175–18.3883]. No significant differences were found in frequencies of the A allele between patients and control groups [OR = 0.7872, 95% CI = 0.4198–1.4762, P = 0.4558]. In addition, there are modest (P 0.05) relationships between serum biochemical parameters levels and rs5746136 genotype in breast cancer patients, but a substantial association between serum SOD activity and GSH concentration and GA and GG rs5746136 genotype in the control group. In conclusion, the current investigation suggests that the AA genotype of (rs5746136) in the MnSOD gene may be associated with an increased risk of breast cancer. The chosen SOD2 variants (rs5746136) play a crucial role in controlling the activity of the SOD enzyme.","PeriodicalId":7529,"journal":{"name":"AIMS Biophysics","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AIMS Biophysics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3934/biophy.2023001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0

Abstract

Oxidative stress factors are among the most common carcinogens, Superoxide dismutase enzyme-2 (SOD2) is an endogenous antioxidant involved in the scavenging of superoxide anions. This study aimed to investigate the effect of the SOD2 gene polymorphism (rs5746136) on SOD activity and biomarker levels in breast cancer patients. This study aimed to investigate the effect of SOD2 gene (rs5746136) polymorphisms on SOD activity and biomarkers levels in breast cancer patients. The spectrophotometry methods were used to detect malondialdehyde (MDA) and Catalase (CAT), Superoxide dismutase (SOD), and Glutathione (GSH) levels, which reflect antioxidant capacity, and the genotypes of rs5746136 were detected utilize PCR and RFLP. According to the current findings, the GA genotype of the control group was the most common (70%), followed by GG and AA genotypes (26.7% and 3.3%) respectively. In the patient group, the most common genotype was GG (45.6%), followed by the GA genotype (42.8%) and then the AA genotype (11.4%) The frequency of heterozygous genotype G/A compared to the homozygous genotype (G/G) [OR = 0.3571, 95% CI = 0.1375–0.9277, P = 0.0345]. The AA genotype is significantly associated with an increased risk of developing BC [OR = 2.00, p = 0.5403, CI: 0.2175–18.3883]. No significant differences were found in frequencies of the A allele between patients and control groups [OR = 0.7872, 95% CI = 0.4198–1.4762, P = 0.4558]. In addition, there are modest (P 0.05) relationships between serum biochemical parameters levels and rs5746136 genotype in breast cancer patients, but a substantial association between serum SOD activity and GSH concentration and GA and GG rs5746136 genotype in the control group. In conclusion, the current investigation suggests that the AA genotype of (rs5746136) in the MnSOD gene may be associated with an increased risk of breast cancer. The chosen SOD2 variants (rs5746136) play a crucial role in controlling the activity of the SOD enzyme.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Rs5746136基因型对乳腺癌患者SOD活性及生物标志物水平的影响
氧化应激因子是最常见的致癌物之一,超氧化物歧化酶-2 (SOD2)是一种内源性抗氧化剂,参与清除超氧阴离子。本研究旨在探讨SOD2基因多态性(rs5746136)对乳腺癌患者SOD活性及生物标志物水平的影响。本研究旨在探讨SOD2基因(rs5746136)多态性对乳腺癌患者SOD活性及生物标志物水平的影响。采用分光光度法检测反映抗氧化能力的丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)水平,并采用PCR和RFLP检测rs5746136基因型。根据目前的研究结果,对照组以GA基因型最常见(70%),其次是GG和AA基因型(26.7%和3.3%)。在患者组中,GG基因型最多(45.6%),其次是GA基因型(42.8%),其次是AA基因型(11.4%)。G/A基因型的杂合子频率与纯合子基因型(G/G)的频率比较[OR = 0.3571, 95% CI = 0.1375 ~ 0.9277, P = 0.0345]。AA基因型与发生BC的风险增加显著相关[OR = 2.00, p = 0.5403, CI: 0.2175-18.3883]。A等位基因频率在患者与对照组间无显著差异[OR = 0.7872, 95% CI = 0.4198 ~ 1.4762, P = 0.4558]。此外,乳腺癌患者血清生化指标水平与rs5746136基因型之间存在适度关系(P < 0.05),而对照组血清SOD活性和GSH浓度与GA和GG rs5746136基因型之间存在显著相关性。综上所述,本研究提示MnSOD基因中(rs5746136)的AA基因型可能与乳腺癌风险增加有关。所选择的SOD2变体(rs5746136)在控制SOD酶的活性方面起着至关重要的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
AIMS Biophysics
AIMS Biophysics BIOPHYSICS-
CiteScore
2.40
自引率
20.00%
发文量
16
审稿时长
8 weeks
期刊介绍: AIMS Biophysics is an international Open Access journal devoted to publishing peer-reviewed, high quality, original papers in the field of biophysics. We publish the following article types: original research articles, reviews, editorials, letters, and conference reports. AIMS Biophysics welcomes, but not limited to, the papers from the following topics: · Structural biology · Biophysical technology · Bioenergetics · Membrane biophysics · Cellular Biophysics · Electrophysiology · Neuro-Biophysics · Biomechanics · Systems biology
期刊最新文献
Endoplasmic reticulum localization of phosphoinositide specific phospholipase C enzymes in U73122 cultured human osteoblasts Identification of potential SARS-CoV-2 papain-like protease inhibitors with the ability to interact with the catalytic triad Predicting factors and top gene identification for survival data of breast cancer A review of molecular biology detection methods for human adenovirus Natural bond orbital analysis of dication magnesium complexes [Mg(H2O)6]2+ and [[Mg(H2O)6](H2O)n]2+; n=1-4
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1