Diversity of Antibiotic-Resistant Escherichia coli from Rivers in Palembang City, South Sumatra, Indonesia

IF 1.6 Q4 ENVIRONMENTAL SCIENCES AIMS Environmental Science Pub Date : 2022-01-01 DOI:10.3934/environsci.2022041
R. N. Sunarti, S. Budiarti, M. Verawaty, Bayo Alhusaeri Siregar, P. Hariani
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Abstract

One of the indicators of water pollution is the presence of coliform bacteria, such as Escherichia coli (E. coli). The development of resistance properties to several antibiotics by this pathogen is a serious health problem. Therefore, this study aims to isolate and identify antibiotic-resistant E. coli using the 16S rRNA gene. Samples were taken along the Kedukan, PU, and Buah rivers in Palembang City, South Sumatra, Indonesia, using the purposive sampling method. Each river consists of 9 sampling points, namely, the lower, middle and the upstream regions. The water quality result for the three rivers show that several water quality parameters do not meet the water quality standards: namely, BOD levels in the Kedukan and Buah rivers, Fe levels in the Kedukan and PU rivers, and chlorine in the three rivers. The results showed that E. coli isolates from the Kedukan, PU and Buah rivers were sensitive to 9 antibiotics. The highest resistance (100%) of E. coli isolates to tobramycin was in the Kedukan and PU rivers, while those from the Buah river were resistant to ampicillin. The phylogenetic tree analysis showed genetic diversity. Two main groups were formed from E. coli, namely, A and B, which consist of 17 and 4 isolates, respectively. Furthermore, the water quality analysis results of the Kedukan, PU, and Buah rivers in Palembang City, South Sumatra, revealed that they are polluted.
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印度尼西亚南苏门答腊岛巨港市河流中耐抗生素大肠杆菌的多样性
水污染的指标之一是大肠菌群的存在,如大肠杆菌(E. coli)。这种病原体对几种抗生素产生耐药性是一个严重的健康问题。因此,本研究旨在利用16S rRNA基因分离和鉴定耐药大肠杆菌。在印度尼西亚南苏门答腊岛巨港市沿Kedukan、PU和Buah河采集样本,采用目的抽样方法。每条河流由9个采样点组成,分别为下游、中游和上游区域。三河水质结果显示,克都干河和布阿河的BOD、克都干河和普河的铁、三河的氯等水质参数均不符合水质标准。结果表明,来自克都坎河、PU河和布阿河的大肠杆菌对9种抗生素敏感。Kedukan河和PU河的大肠杆菌对妥布霉素的耐药性最高(100%),而Buah河的大肠杆菌对氨苄青霉素的耐药性最高。系统发育树分析显示遗传多样性。大肠杆菌形成两个主要类群,即A和B,分别有17株和4株菌株。此外,南苏门答腊岛巨港市的Kedukan河、PU河和Buah河的水质分析结果显示,它们受到污染。
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来源期刊
AIMS Environmental Science
AIMS Environmental Science ENVIRONMENTAL SCIENCES-
CiteScore
2.90
自引率
0.00%
发文量
31
审稿时长
5 weeks
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