Taking Advantage of Taguchi Design Method to Optimize Medium Culture Conditions for Producing Recombinant Follicle Stimulating Hormone

Abstract

Medium culture optimization is an Effective, available and financially affordable way to improve production of recombinant proteins produced by genetic engineering. The existence of varieties of parameters and different levels for each, makes it complex, time-consuming and expensive to determine the optimum point of all parameters by applying the factorial method. To overcome these difficulties, in this study, Taguchi robust design method was employed. The environmental parameter such as temperature, pH and glutamine concentration in 4 different levels were considered. According to the design of the experiments, FSH titer was measured. In comparison with the control condition, 14.92 fold overexpression was observed. The best level for these parameters was pH=7.0, 28°C and 2 mM Glutamine concentration. Citation: Qafary M, Gharanfoli M, Qafari SM (2018) Taking Advantage of Taguchi Design Method to Optimize Medium Culture Conditions for Producing Recombinant Follicle Stimulating Hormone. J Biom Biostat 9: 409. doi: 10.4172/2155-6180.1000409