Characterization of Bone Marrow-Derived Dendritic Cells Developed in Serum-Free Media and their Ability to Prevent Type 1 Diabetes in Nonobese Diabetic Mice

Benjamin M. Looney, Anna V. Chernatynskaya, M. Clare-Salzler, C. Xia
{"title":"Characterization of Bone Marrow-Derived Dendritic Cells Developed in Serum-Free Media and their Ability to Prevent Type 1 Diabetes in Nonobese Diabetic Mice","authors":"Benjamin M. Looney, Anna V. Chernatynskaya, M. Clare-Salzler, C. Xia","doi":"10.4172/2155-9864.1000206","DOIUrl":null,"url":null,"abstract":"Dendritic cells (DC) have been investigated as a cell-based therapy for Type 1 Diabetes (T1D). BM-DC expanded ex vivo with GM-CSF and IL-4 is typically cultured with fetal bovine serum (FBS). The effect of FBS on NOD BM-DC has not been extensively studied. In the present study we compare BM-DC generated in serum-free culture media (X-VIVO20; FBS−) with BM-DC generated in media containing 10% FBS (RPMI1640/10%FBS; FBS+). We show that FBS− BM-DC display a phenotype and cytokine-producing profile distinct from FBS+ BMDC. Additionally, compared to FBS+ BM-DC, we show evidence of an altered Th cell response induced by FBS− BM-DC. Finally, we demonstrate that only FBS− BM-DC prevent the onset of T1D and induce increased levels of CD4+Foxp3+ regulatory T cells as well as a long-lasting β cell-specific T cell response. This study indicates that serum-free media generates a more tolerogenic BM-DC capable of preventing T1D in the NOD mice.","PeriodicalId":73627,"journal":{"name":"Journal of blood disorders & transfusion","volume":"5 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2014-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2155-9864.1000206","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of blood disorders & transfusion","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2155-9864.1000206","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

Dendritic cells (DC) have been investigated as a cell-based therapy for Type 1 Diabetes (T1D). BM-DC expanded ex vivo with GM-CSF and IL-4 is typically cultured with fetal bovine serum (FBS). The effect of FBS on NOD BM-DC has not been extensively studied. In the present study we compare BM-DC generated in serum-free culture media (X-VIVO20; FBS−) with BM-DC generated in media containing 10% FBS (RPMI1640/10%FBS; FBS+). We show that FBS− BM-DC display a phenotype and cytokine-producing profile distinct from FBS+ BMDC. Additionally, compared to FBS+ BM-DC, we show evidence of an altered Th cell response induced by FBS− BM-DC. Finally, we demonstrate that only FBS− BM-DC prevent the onset of T1D and induce increased levels of CD4+Foxp3+ regulatory T cells as well as a long-lasting β cell-specific T cell response. This study indicates that serum-free media generates a more tolerogenic BM-DC capable of preventing T1D in the NOD mice.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
无血清培养基中培养的骨髓来源树突状细胞的特性及其在非肥胖糖尿病小鼠中预防1型糖尿病的能力
树突状细胞(DC)作为一种基于细胞的治疗1型糖尿病(T1D)的方法已被研究。用GM-CSF和IL-4体外扩增的BM-DC通常与胎牛血清(FBS)一起培养。FBS对NOD BM-DC的影响尚未得到广泛研究。在本研究中,我们比较了无血清培养基(X-VIVO20;FBS−),在含有10%FBS的培养基中生成BM-DC (RPMI1640/10%FBS;的边后卫+)。我们发现FBS - BM-DC表现出与FBS+ BMDC不同的表型和细胞因子产生谱。此外,与FBS+ BM-DC相比,我们发现FBS - BM-DC诱导的Th细胞反应发生了改变。最后,我们证明了只有FBS - BM-DC可以预防T1D的发生,并诱导CD4+Foxp3+调节性T细胞水平的增加,以及持久的β细胞特异性T细胞反应。本研究表明,无血清培养基可在NOD小鼠中产生耐受性更强的BM-DC,能够预防T1D。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
FOR LIVER: Herbs in Pakistani Population Urinary Sickle Cells in a Patient with Vaso-Occlusive Crisis: A Case Report Dengue Hemorrhagic Fever and Non Immune Hemolytic Anemia: Two Recherché in Alliance Computer-Assisted Aneurysm Growth Evaluation and Detection (AGED): Comparison with Clinical Aneurysm Follow-Up. Iron-Deficiency Anemias Worsen Some Cardiovascular Diseases: The Role of Intravenous Ferric Carboxymaltose
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1