Rigid Ryegrass (Lolium rigidum Gaud) Resistant to ACCase and ALS inhibitors in northeastern Iran

IF 1.8 4区 农林科学 Q2 PLANT SCIENCES Advances in Weed Science Pub Date : 2023-06-30 DOI:10.51694/advweedsci/2023;41:00008
A. Tavassoli, J. Gherekhloo, F. Ghaderi‐Far, E. Zand, M. D. Osuna, R. Prado
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Abstract

: Background: Among the weeds in Iran, resistant Lolium rigidum Gaud is considered a troublesome weed in winter cereals due to its tendency to evolve cross (CR) and multiple resistance (MR) to herbicides. Objective: This research examined the patterns and mechanisms of L. rigidum resistance to clodinafop-propargyl (CP) and mesosulfuron methyl+iodosulfuron methyl (MI). Methods: Experiments were conducted on four putative-resistant L. rigidum biotypes and one susceptible biotype. The dose-response assay was performed on the biotypes with CP and MI. CR and MR were investigated with haloxyfop-R-methyl (HRM), sethoxydim (SD), pinoxaden (PN). and isoproturon+ diflufenican (ID) herbicides. An indirect study of the metabolism of herbicides was carried out using the cytochrome P450 monooxygenase (CYP450) inhibitors 1-aminobenzotriazole (ABT), piperonyl butoxide (PBO) and malathion. Finally, sequencing of ALS and ACCase genes was performed to investigate target-site resistance. Results: All putative-resistant L. rigidum biotypes were resistant to CP, MI, and HRM, but susceptible to SD, PN, and ID. The indirect study showed that the P450 enzyme had no role in the evolution of resistance in L. rigidum biotypes. Resistance in this species was due to Ile-1781-Leu and Pro-197-Ser substitutions on ACCase and ALS encoding genes led to resistance, respectively. Conclusions: Resistance in the studied L. rigidum biotypes to ALS and ACCase inhibiting was due of target site resistance. If these resistant biotypes are not controlled, they will become a problem for farmers in the region
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伊朗东北部坚硬黑麦草(Lolium rigum Gaud)对ACCase和ALS抑制剂的抗性
背景:在伊朗的杂草中,由于对除草剂具有交叉抗性(CR)和多重抗性(MR)的倾向,抗性稻瘟病(Lolium rigidum Gaud)被认为是冬季谷物中的麻烦杂草。前言:目的:研究松鸡对氯地那福丙炔(CP)和甲基中磺隆+甲基碘磺隆(MI)的抗性模式和机制。方法:对4种推定抗性菌和1种敏感菌进行试验。用CP和MI对生物型进行剂量反应测定,用haloxyfop-R-methyl (HRM)、sethoxydim (SD)、pinoxaden (PN)对CR和MR进行测定。异丙醇+双氟虫腈(ID)除草剂。采用细胞色素P450单加氧酶(CYP450)抑制剂1-氨基苯并三唑(ABT)、胡椒酰丁醇(PBO)和马拉硫磷对除草剂的代谢进行了间接研究。最后,对ALS和ACCase基因进行测序以研究靶点耐药性。结果:所有假定耐药的刚性乳杆菌生物型均对CP、MI和HRM耐药,但对SD、PN和ID敏感。间接研究表明P450酶在僵菌生物型的抗性进化中没有作用。该品种的抗性是由于ACCase编码基因上的Ile-1781-Leu和Pro-197-Ser分别被取代而产生的。结论:僵菌对ALS和ACCase抑制的抗性是由靶位抗性引起的。如果这些抗药生物型得不到控制,它们将成为该地区农民的一个问题
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来源期刊
Advances in Weed Science
Advances in Weed Science PLANT SCIENCES-
CiteScore
2.10
自引率
42.90%
发文量
25
期刊最新文献
Shortening critical period of weed control at soybean by residual herbicide mixtures Introduction and management of Amaranthus palmeri in Brazil Rigid Ryegrass (Lolium rigidum Gaud) Resistant to ACCase and ALS inhibitors in northeastern Iran Use of inhibitors and protectors in the tolerance of white oat cultivars to the penoxsulam herbicide The effect of environmental factors on seed germination and emergence of cutleaf geranium
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