Attenuation of cisplatin-induced nephrotoxicity by ethanol extract of seeds of Lens culinaris medik

A. Sreedevi, Kaveripakam Saisruthi
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引用次数: 2

Abstract

Aim: The present study was undertaken to evaluate the potential role of ethanol extract of seeds of Lens culinaris in attenuation of Cisplatin-induced nephrotoxicity in male Albino rats. Materials and Methods: Seeds of Lens culinaris were powdered, defatted and ethanol extract was prepared by hot extraction method. The ethanol extract so prepared was subjected to preliminary phytochemical evaluation and fingerprint analysis using high performance thin layer chromatography. Nephroprotective potential of extract was evaluated at 200 and 400 mg/kg b.w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intra peritoneal injection of Cisplatin at a dose of 5 mg/kg b.w. Nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels in renal tissue. Results: Cisplatin had induced marked acute renal toxicity as manifested by a significant increase in serum marker levels, urinary total protein, lipid peroxidation and decrease in urinary creatinine, catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH). The administration of extract significantly attenuated the Cisplatin-induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in a dose dependent manner. Histological and immunohistochemical studies also substantiated the biochemical studies. Conclusion: The findings of the present study provides corroborative scientific evidence for the attenuation of Cisplatin-induced nephrotoxicity by seeds of Lens culinaris and validates the ethno-medicinal use of this seeds as renoprotective agent.
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水芹籽乙醇提取物对顺铂所致肾毒性的抑制作用
目的:探讨黄芪种子乙醇提取物对雄性白化大鼠顺铂肾毒性的抑制作用。材料与方法:采用热浸法制备水仙种子粉化、脱脂、乙醇提取物。采用高效薄层色谱法对所制备的乙醇提取物进行初步的植物化学评价和指纹图谱分析。研究了200和400 mg/kg b.w.提取物对雄性白化病大鼠的肾保护作用。单次腹腔注射顺铂5 mg/kg b.w引起肾毒性,通过测定血清标志物、尿参数、肾组织脂质过氧化和抗氧化水平评估肾保护活性。结果:顺铂引起了明显的急性肾毒性,表现为血清标志物水平、尿总蛋白、脂质过氧化水平显著升高,尿肌酐、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、还原性谷胱甘肽(GSH)水平显著降低。在治疗和预防方案中,提取物通过恢复生化和氧化应激标志物,以剂量依赖的方式显著减轻顺铂诱导的肾毒性。组织学和免疫组织化学研究也证实了生化研究。结论:本研究结果为黄连种子对顺铂所致肾毒性的衰减提供了有力的科学依据,验证了黄连种子作为肾保护剂的民族药用价值。
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