Marker assay guided standardization of an ayurvedic concentrated polyherbal decoction “CiruvilvāDi KaṣāYam” and its application in industrial quality control

H. Purushothaman, Ancily Davis, Migy John, Meerabai Palazhy Kalarikkal, A. Bhaskaran, Basil Eldho, T. Antony
{"title":"Marker assay guided standardization of an ayurvedic concentrated polyherbal decoction “CiruvilvāDi KaṣāYam” and its application in industrial quality control","authors":"H. Purushothaman, Ancily Davis, Migy John, Meerabai Palazhy Kalarikkal, A. Bhaskaran, Basil Eldho, T. Antony","doi":"10.4103/asl.ASL_188_17","DOIUrl":null,"url":null,"abstract":"Background: To establish a marker guided standardization technique to assess whether the marketed concentrated kaṣāyam (decoction) prepared in large scale is providing the same phytochemical values of a Laboratory Reference Standard (LRS) kaṣāyam prepared classically from raw materials of Pharmacopoeial quality. Objectives: Manufacturing and standardization of LRS 'Ciruvilvādi kaṣāyam', including marker assays and its comparison with four different marketed samples. Materials and Methods: pH, Brix and Total solids of samples were determined and compared. HPTLC profile comparison and quantitative comparison with HPLC were done with Gallic acid and Piperine as standards. Results and Conclusion: Results of Brix and Total solids imply that, the manufacturing process of sample IV was significantly different from other samples and LRS. pH value of sample III showed a significant difference as compared to other samples and LRS, indicating a difference in phytochemical contents. Quantification of Piperine and Gallic acid revealed that sample III has a very low Gallic acid concentration and zero Piperine concentration. Sample IV showed a very high concentration of Gallic acid when compared to other samples and LRS. Sample I, II and IV had piperine content but significantly lower when compared to the LRS. In conclusion, the samples (1-IV) which showed difference with the LRS should be made phytochemically comparable to in-house LRS or Pharmacopoeial standards by adopting the marker assay standardization technique. This method helps to identify and rectify the problems related to raw material, in process and finished product quality control.","PeriodicalId":7805,"journal":{"name":"Ancient Science of Life","volume":"44 1","pages":"24 - 30"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ancient Science of Life","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/asl.ASL_188_17","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background: To establish a marker guided standardization technique to assess whether the marketed concentrated kaṣāyam (decoction) prepared in large scale is providing the same phytochemical values of a Laboratory Reference Standard (LRS) kaṣāyam prepared classically from raw materials of Pharmacopoeial quality. Objectives: Manufacturing and standardization of LRS 'Ciruvilvādi kaṣāyam', including marker assays and its comparison with four different marketed samples. Materials and Methods: pH, Brix and Total solids of samples were determined and compared. HPTLC profile comparison and quantitative comparison with HPLC were done with Gallic acid and Piperine as standards. Results and Conclusion: Results of Brix and Total solids imply that, the manufacturing process of sample IV was significantly different from other samples and LRS. pH value of sample III showed a significant difference as compared to other samples and LRS, indicating a difference in phytochemical contents. Quantification of Piperine and Gallic acid revealed that sample III has a very low Gallic acid concentration and zero Piperine concentration. Sample IV showed a very high concentration of Gallic acid when compared to other samples and LRS. Sample I, II and IV had piperine content but significantly lower when compared to the LRS. In conclusion, the samples (1-IV) which showed difference with the LRS should be made phytochemically comparable to in-house LRS or Pharmacopoeial standards by adopting the marker assay standardization technique. This method helps to identify and rectify the problems related to raw material, in process and finished product quality control.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
标记法指导阿育吠陀浓缩复方汤剂CiruvilvāDi KaṣāYam的标准化及其在工业质量控制中的应用
背景:建立一种以标记为导向的标准化技术,以评估市场上大规模制备的浓缩kaṣāyam(煎剂)是否与药典质量原料经典制备的实验室参考标准kaṣāyam具有相同的植物化学值。目的:LRS 'Ciruvilvādi kaṣāyam'的生产和标准化,包括标记物测定及其与四种不同上市样品的比较。材料与方法:测定样品的pH值、糖度和总固形物。以没食子酸和胡椒碱为对照品,分别进行HPLC和HPLC图谱比较。结果与结论:白锐度和总固形物的测定结果表明,样品IV的制作工艺与其他样品和LRS有显著差异。样品III的pH值与其他样品和LRS相比存在显著差异,说明植物化学物质含量存在差异。胡椒碱和没食子酸的定量分析表明,样品III的没食子酸浓度很低,胡椒碱浓度为零。与其他样品和LRS相比,样品IV显示出非常高的没食子酸浓度。样品I、II和IV的胡椒碱含量明显低于LRS。综上所述,对与LRS存在差异的样品(1-IV),应采用标记物分析标准化技术,使其在植物化学上与国内LRS或药典标准具有可比性。该方法有助于识别和纠正与原材料、过程和成品质量控制有关的问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
39 weeks
期刊最新文献
Vaidyaka Paribhasha Pradipa: A treatise on technical terminologies of Bhaishajya Kalpana (Ayurvedic Pharmaceutics) Herbal emulgel containing Azadirachta indica (Neem) and Nigella Sativa L. (Black Cumin) oils in wound management: Preclinical investigations Quantitative estimation of secondary metabolites of shadanga paniya classical ayurvedic formulation and constituent herbs Editorial note – Resurgent Ayurveda and resurging research on Ayurveda A proposal for “Ayurveda and informatics” as a modular course in BTech engineering program
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1