Luwei Tian Luwei Tian, Ming Guo Ming Guo, Xingtao Xu and Linfang Shi Xingtao Xu and Linfang Shi
{"title":"Imaging Serum Proteome Behavior in Process of Lead Transportation in Vivo: A Fluorescence Spectroscopic Analysis Insight","authors":"Luwei Tian Luwei Tian, Ming Guo Ming Guo, Xingtao Xu and Linfang Shi Xingtao Xu and Linfang Shi","doi":"10.52568/001122/jcsp/44.05.2022","DOIUrl":null,"url":null,"abstract":"As a common heavy metal, Pb (II) can react with biological macromolecules in the human body and have an impact on human health, but there are few studies on its synergistic interaction with a variety of proteins of different abundance. Pb (II) binding with a synthetic model protein system (bovine serum albumin (BSA) and bovine lactoferrin (BLF)) was characterized using fluorescence spectroscopy and was described using a quantitative model. Pb (II) quenched the fluorescence of BSA-BLF, indicating that Pb (II) interacted with the BSA-BLF protein system, and was affected by single protein, mixed proteins and the solution microenvironment. A model was constructed and an indicator of the interaction (FPI) was derived to quantify the interactions. There was a high correlation (R2=0.9182, pandlt;0.001) between the FPI and the Pb (II) concentration when the interaction models were analyzed with a Taylor function. The effects of the solvent microenvironment on BSA, BLF and BSA-BLF were evaluated using the IOM (overall microenvironmental influence factor). BLF was more easily affected by the solution microenvironment with increasing concentrations than BSA, while BSA-BLF was less affected.","PeriodicalId":17253,"journal":{"name":"Journal of the chemical society of pakistan","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the chemical society of pakistan","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.52568/001122/jcsp/44.05.2022","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
As a common heavy metal, Pb (II) can react with biological macromolecules in the human body and have an impact on human health, but there are few studies on its synergistic interaction with a variety of proteins of different abundance. Pb (II) binding with a synthetic model protein system (bovine serum albumin (BSA) and bovine lactoferrin (BLF)) was characterized using fluorescence spectroscopy and was described using a quantitative model. Pb (II) quenched the fluorescence of BSA-BLF, indicating that Pb (II) interacted with the BSA-BLF protein system, and was affected by single protein, mixed proteins and the solution microenvironment. A model was constructed and an indicator of the interaction (FPI) was derived to quantify the interactions. There was a high correlation (R2=0.9182, pandlt;0.001) between the FPI and the Pb (II) concentration when the interaction models were analyzed with a Taylor function. The effects of the solvent microenvironment on BSA, BLF and BSA-BLF were evaluated using the IOM (overall microenvironmental influence factor). BLF was more easily affected by the solution microenvironment with increasing concentrations than BSA, while BSA-BLF was less affected.
期刊介绍:
This journal covers different research areas in the field of Chemistry. These include; Analytical Chemistry, Applied Chemistry, Biochemistry, Environmental Chemistry, Industrial Chemistry, Inorganic Chemistry, Organic Chemistry and Physical Chemistry. The journal publishes full length articles and Reviews from researchers in academia in addition to featuring comments. Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry.