Effect of piper Crocatum leaves extract on atherosclerosis in diabetic rats

Q3 Biochemistry, Genetics and Molecular Biology Journal of Advanced Biotechnology and Experimental Therapeutics Pub Date : 2023-01-01 DOI:10.5455/jabet.2023.d123
Muhammad Adiansyah, Endang Mahati, F. Mundhofir, Nani Maharani, Y. Nindita, H. Istiadi
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Abstract

Atherosclerosis is caused by an inflammatory process in the endothelium due to an imbalance between oxidant and antioxidant agents that often occurs in Diabetes mellitus (DM) patients. Antioxidants that function to neutralize superoxide can be used to prevent atherosclerosis in DM. Piper crocatum leaves shows an unknown antioxidant effect on the atherosclerosis process.The aim of this study is to determine the effect Piper crocatum leaves extract on blood MDA levels and the severity of atherosclerosis in diabetic rats. 30 Sprague Dawley rats were randomly divided into 6 groups, namely, HC (nondiabetic), NC (diabetic-no therapy), PC (metformin 45 mg/kg), PC200, PC300, PC400 (Piper crocatum leaves extract 200/300/ 400 mg/kg/day). Termination, blood sampling, and histopathological analysis were performed after 14 days of treatment. MDA was measured using the TBARS method, and read on a 532 nm wavelength UV-vis spectrophotometer. The severity of atherosclerosis of the rat carotid artery was observed from tissue stained with Haematoxylin Eosin staining at 400x microscope magnification. The mean blood MDA levels in the PC200, PC300, PC400 groups were 1.24 ± 0.26, 0.85 ± 0.35, 0.10 and ± 0.02, respectively, and a decrease in MDA levels was found in the PC200, PC300, and PC400 compared to group NC. The study showed a decrease in the severity of atherosclerosis in groups PC200, PC300, PC400 compared to groups NC. Piper crocatum leaves extract had an effect on decreasing MDA levels and severity of atherosclerosis in diabetic rats.
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藏红花叶提取物对糖尿病大鼠动脉粥样硬化的影响
动脉粥样硬化是由内皮细胞炎症过程引起的,这是由于氧化剂和抗氧化剂之间的不平衡引起的,这种情况经常发生在糖尿病(DM)患者中。具有中和超氧化物功能的抗氧化剂可用于预防糖尿病动脉粥样硬化。风笛草叶对动脉粥样硬化过程显示出未知的抗氧化作用。本研究旨在探讨虎笛叶提取物对糖尿病大鼠血MDA水平及动脉粥样硬化严重程度的影响。将30只Sprague Dawley大鼠随机分为6组,即HC(非糖尿病组)、NC(糖尿病-未治疗组)、PC(二甲双胍45 mg/kg组)、PC200、PC300、PC400(虎耳叶提取物200/300/ 400 mg/kg/d组)。治疗14天后进行终止治疗、抽血和组织病理学分析。采用TBARS法测定MDA,并在532 nm波长紫外-可见分光光度计上读取。在400倍显微镜下,用红木精伊红染色观察大鼠颈动脉粥样硬化的严重程度。PC200、PC300、PC400组血MDA均值分别为1.24±0.26、0.85±0.35、0.10、±0.02,PC200、PC300、PC400组MDA均较NC组降低。研究显示,与NC组相比,PC200、PC300、PC400组的动脉粥样硬化严重程度有所降低。虎笛叶提取物具有降低糖尿病大鼠动脉粥样硬化严重程度和丙二醛水平的作用。
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来源期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
Journal of Advanced Biotechnology and Experimental Therapeutics Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.90
自引率
0.00%
发文量
41
审稿时长
8 weeks
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