Nisreen W. Mustafa, Ola Naser, Zaid N. Elia, Sanaria F. Jarjes
Progranulin (PGRN), is a multifunctional protein with profound expression in epithelial and immune cells in which plays a crucial role in controlling host-defense signaling pathways during infection and inflammation. The current study carried out to evaluate the efficiency of progranulin as a predictive inflammatory marker for a group of diseases with different etiologies that cause acute and chronic inflammations. A total of 120 participants with various diseases (rheumatoid arthritis (RA), Helicobacter pylori infection, burn wound, hepatitis B and prostate cancer) in addition to 20 healthy people were enrolled in this study. The levels of serum PGRN and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA), while chemiluminescent microparticle immunoassay (CMIA) was used to detect the concentrations of high-sensitive C-reactive protein (hsCRP). Elevated serum PGRN levels have been reported in all patient groups when compared with those in healthy controls (P > 0.05). Likewise, increased serum hsCRP and IL-6 levels were seen in all patient groups. However, for some patient groups, the differences in hsCRP and IL-6 levels did not reach statistical significance (P > 0.05) in comparison with control group. Furthermore, serum PGRN levels exhibited positive correlation with hsCRP in H. pylori and RA patient groups. As well as, with IL-6 only in RA patient group, whereas no significant correlations were found with the rest of studied diseases. This study concluded that progranulin is an effective nonspecific inflammatory indicator of acute and chronic inflammations. It had also a higher predictive efficiency than hsCRP and IL-6 which are commonly used as inflammatory predictive markers.
{"title":"Evaluation of the inflammatory predictive efficiency of progranulin as compared with common pro-inflammatory regulators","authors":"Nisreen W. Mustafa, Ola Naser, Zaid N. Elia, Sanaria F. Jarjes","doi":"10.5455/jabet.2023.d128","DOIUrl":"https://doi.org/10.5455/jabet.2023.d128","url":null,"abstract":"Progranulin (PGRN), is a multifunctional protein with profound expression in epithelial and immune cells in which plays a crucial role in controlling host-defense signaling pathways during infection and inflammation. The current study carried out to evaluate the efficiency of progranulin as a predictive inflammatory marker for a group of diseases with different etiologies that cause acute and chronic inflammations. A total of 120 participants with various diseases (rheumatoid arthritis (RA), Helicobacter pylori infection, burn wound, hepatitis B and prostate cancer) in addition to 20 healthy people were enrolled in this study. The levels of serum PGRN and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA), while chemiluminescent microparticle immunoassay (CMIA) was used to detect the concentrations of high-sensitive C-reactive protein (hsCRP). Elevated serum PGRN levels have been reported in all patient groups when compared with those in healthy controls (P > 0.05). Likewise, increased serum hsCRP and IL-6 levels were seen in all patient groups. However, for some patient groups, the differences in hsCRP and IL-6 levels did not reach statistical significance (P > 0.05) in comparison with control group. Furthermore, serum PGRN levels exhibited positive correlation with hsCRP in H. pylori and RA patient groups. As well as, with IL-6 only in RA patient group, whereas no significant correlations were found with the rest of studied diseases. This study concluded that progranulin is an effective nonspecific inflammatory indicator of acute and chronic inflammations. It had also a higher predictive efficiency than hsCRP and IL-6 which are commonly used as inflammatory predictive markers.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aims to identify the relationship between oxidative stress, vitamin B12, Homocysteine, and DNA methylation to evaluate their role in the progression of B.C. disease. Methodology: The 5mC global DNA methylation levels in 60 women diagnosed with breast cancer (age range 33–80 yrs.) and 30 age-matched healthy controls were assessed using Methyl Flash™ Methylated DNA Quantification Kit. Patients with B.C. were divided into two groups: group 1 consisted of stage II breast cancer women (Low level), and Group 2 consisted of patients in stage III and IV (High level). Malondialdehyde (MDA), Glutathione peroxidase (GPX), Homocysteine (HCY) and Vitamin B12 levels in the studied subjects were detected by measuring using ELISA. Results: The results showed a significant increase of HCY and MDA in breast cancer patients compared to healthy control, with apparent increases in patients with breast cancer in an advanced stage. They were accompanied by significantly reduced levels of 5mC with a positive correlation between 5mC and different stages of B.C. Also, the results showed that patients in advanced settings and those with a poor prognosis were exposed to low levels of Vit. B12 and GPX (except the patient in stage IV showed increased GPX level). Conclusion: In conclusion, our findings suggest an involvement of Glutathione peroxidase and Homocysteine in the progression of breast cancer, with the potential of utilizing the differences in the levels of global DNA methylation at the different stages as a risk factor for developing the breast cancer.
{"title":"Evaluation of oxidative stress activity and the levels of homocysteine, vitamin B12, and DNA methylation among women with breast cancer","authors":"Rana Rubaye, Rakad Jumaily","doi":"10.5455/jabet.2023.d114","DOIUrl":"https://doi.org/10.5455/jabet.2023.d114","url":null,"abstract":"Objective: This study aims to identify the relationship between oxidative stress, vitamin B12, Homocysteine, and DNA methylation to evaluate their role in the progression of B.C. disease. Methodology: The 5mC global DNA methylation levels in 60 women diagnosed with breast cancer (age range 33–80 yrs.) and 30 age-matched healthy controls were assessed using Methyl Flash™ Methylated DNA Quantification Kit. Patients with B.C. were divided into two groups: group 1 consisted of stage II breast cancer women (Low level), and Group 2 consisted of patients in stage III and IV (High level). Malondialdehyde (MDA), Glutathione peroxidase (GPX), Homocysteine (HCY) and Vitamin B12 levels in the studied subjects were detected by measuring using ELISA. Results: The results showed a significant increase of HCY and MDA in breast cancer patients compared to healthy control, with apparent increases in patients with breast cancer in an advanced stage. They were accompanied by significantly reduced levels of 5mC with a positive correlation between 5mC and different stages of B.C. Also, the results showed that patients in advanced settings and those with a poor prognosis were exposed to low levels of Vit. B12 and GPX (except the patient in stage IV showed increased GPX level). Conclusion: In conclusion, our findings suggest an involvement of Glutathione peroxidase and Homocysteine in the progression of breast cancer, with the potential of utilizing the differences in the levels of global DNA methylation at the different stages as a risk factor for developing the breast cancer.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Tanim, S. Shoily, T. Ahsan, K. Fatema, Sarah Mahdiyah, A. Sajib
Vascular Endothelial Growth Factor A (VEGFA) is a glycoprotein that mediates various biological processes, including angiogenesis, vascular permeability, and cellular migration. Aberrant VEGFA signaling is also one of the hallmarks of many types of cancer and has been implicated in various ophthalmological conditions such as diabetic macular edema and age-related macular degeneration. Consequently, a number of therapeutic monoclonal antibodies (mAb) targeting VEGFA have been developed and are widely used to treat these conditions. Bevacizumab (BVZ) and Ranibizumab (RBZ) are two such antibodies that are commercially available and used to treat various cancers and ophthalmological conditions. Nevertheless, a very high rate of non-responsiveness to these mAb treatments has been reported. Therefore, it is important to predict the response to these therapeutic mAb treatments in patients in a personalized approach. This study was aimed at analyzing the impacts of missense variants in the respective VEGFA epitopes for these two therapeutic anti-VEGFA mAbs (BVZ and RBZ) on their interaction with VEGFA through the use of multiple in silico tools. Three missense variants (VEGFAR82W, VEGFAR82Q, and VEGFAG92R) in VEGFA epitopes appear to significantly destabilize VEGFA-BVZ interaction, while only two variants (VEGFAR82W and VEGFAR82Q) affect interaction of VEGFA with RBZ. The VEGFAR82W variant may be pathogenic as well. These missense variants may play roles in the observed heterogeneous response to anti-VEGFA mAb treatments in patients and, therefore, may be used as pharmacogenetic markers for the prediction of responses before administration, and thus for the improvement of therapeutic outcomes.
{"title":"Interaction of epitopic missense variants of VEGFA with therapeutic monoclonal antibodies","authors":"M. Tanim, S. Shoily, T. Ahsan, K. Fatema, Sarah Mahdiyah, A. Sajib","doi":"10.5455/jabet.2023.d132","DOIUrl":"https://doi.org/10.5455/jabet.2023.d132","url":null,"abstract":"Vascular Endothelial Growth Factor A (VEGFA) is a glycoprotein that mediates various biological processes, including angiogenesis, vascular permeability, and cellular migration. Aberrant VEGFA signaling is also one of the hallmarks of many types of cancer and has been implicated in various ophthalmological conditions such as diabetic macular edema and age-related macular degeneration. Consequently, a number of therapeutic monoclonal antibodies (mAb) targeting VEGFA have been developed and are widely used to treat these conditions. Bevacizumab (BVZ) and Ranibizumab (RBZ) are two such antibodies that are commercially available and used to treat various cancers and ophthalmological conditions. Nevertheless, a very high rate of non-responsiveness to these mAb treatments has been reported. Therefore, it is important to predict the response to these therapeutic mAb treatments in patients in a personalized approach. This study was aimed at analyzing the impacts of missense variants in the respective VEGFA epitopes for these two therapeutic anti-VEGFA mAbs (BVZ and RBZ) on their interaction with VEGFA through the use of multiple in silico tools. Three missense variants (VEGFAR82W, VEGFAR82Q, and VEGFAG92R) in VEGFA epitopes appear to significantly destabilize VEGFA-BVZ interaction, while only two variants (VEGFAR82W and VEGFAR82Q) affect interaction of VEGFA with RBZ. The VEGFAR82W variant may be pathogenic as well. These missense variants may play roles in the observed heterogeneous response to anti-VEGFA mAb treatments in patients and, therefore, may be used as pharmacogenetic markers for the prediction of responses before administration, and thus for the improvement of therapeutic outcomes.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jennifer Faustin, N. Susilaningsih, M. Muniroh, B. Bahrudin, Endang Mahati, H. Istiadi
Atherosclerosis is a complex pathological process begins with endothelial dysfunction, one of which is triggered by an inflammatory process due to increased levels of low-density lipoprotein (LDL) cholesterol and exposure to cigarette smoke. White tea leaf (Camellia sinensis) shows an anti-inflammatory effect which has not been known for its effect on the atherosclerotic process. The aim of this study is to determine the effect of Camellia sinensis leaf extract on IL-6 levels, foam cell count, and the ratio of intima-media thickness of cigarette smoke and high-fat diet-induced atherosclerosis in Wistar rats. Thirty male Wistar rats were randomly divided into 5 groups namely HC (healthy control), NC (negative control), WT100, WT200, and WT400 (treated with white tea leaf extract 100, 200, and 400 mg/kgBW/day, respectively). IL-6 levels were measured by the ELISA method. At 400x microscope magnification, foam cell count and intima-media thickness ratio were seen in aortic tissue. Administration of graded doses of white tea leaf extract in groups WT100, WT200, WT400 significantly reduced IL-6 levels, foam cell count, and intima-media thickness ratio of abdominal aorta compared to the NC group. These results suggest that white tea leaf extract may decrease levels of IL-6, foam cell count, and intima-media thickness ratio in atherosclerosis-induced Wistar rats.
{"title":"Effect of white tea (Camellia sinensis) leaf extract on cigarette smoke and high-fat diet-induced atherosclerosis in Wistar rats","authors":"Jennifer Faustin, N. Susilaningsih, M. Muniroh, B. Bahrudin, Endang Mahati, H. Istiadi","doi":"10.5455/jabet.2023.d148","DOIUrl":"https://doi.org/10.5455/jabet.2023.d148","url":null,"abstract":"Atherosclerosis is a complex pathological process begins with endothelial dysfunction, one of which is triggered by an inflammatory process due to increased levels of low-density lipoprotein (LDL) cholesterol and exposure to cigarette smoke. White tea leaf (Camellia sinensis) shows an anti-inflammatory effect which has not been known for its effect on the atherosclerotic process. The aim of this study is to determine the effect of Camellia sinensis leaf extract on IL-6 levels, foam cell count, and the ratio of intima-media thickness of cigarette smoke and high-fat diet-induced atherosclerosis in Wistar rats. Thirty male Wistar rats were randomly divided into 5 groups namely HC (healthy control), NC (negative control), WT100, WT200, and WT400 (treated with white tea leaf extract 100, 200, and 400 mg/kgBW/day, respectively). IL-6 levels were measured by the ELISA method. At 400x microscope magnification, foam cell count and intima-media thickness ratio were seen in aortic tissue. Administration of graded doses of white tea leaf extract in groups WT100, WT200, WT400 significantly reduced IL-6 levels, foam cell count, and intima-media thickness ratio of abdominal aorta compared to the NC group. These results suggest that white tea leaf extract may decrease levels of IL-6, foam cell count, and intima-media thickness ratio in atherosclerosis-induced Wistar rats.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yance Kaitana, N. Kurnia, M. Tulung, J. Tuda, J. Mamahit, T. Tallei
Aedes spp. are the primary vectors of arboviruses such as dengue, Zika, and chikungunya. To date neither an approved vaccine nor a drug that can definitively prevent or treat these diseases, vector control continues to be an essential method of disease prevention. Plant-based insecticides are an alternative to chemical insecticides because they are less toxic to non-target insects and degrade more quickly. The Papuan people have used Pangi leaves for generations as a potent natural chemical against head lice and mosquito larvae. This study aimed to evaluate the potential of pangi (Pangium edule) leaf extract as a biolarvicide and mosquito repellent. Fresh pangi leaves were extracted with water by using a blender to pulverize the leaves. One-way ANOVA was used to examine the variance in mortality rates, and the LC50 value was calculated for probit analysis. Dead larvae were identified and counted. In addition to testing the effectiveness of the extracts as biolarvicide, the extract was also tested as potential insect repellents. The findings demonstrated that pangi leaf extract can kill mosquito larvae. The concentration of the extract affected larval mortality. Furthermore, the extract demonstrated its effectiveness as an insect repellent. Pangi leaf extract contains alkaloids, flavonoids, tannins, saponins, phenolic compounds, and cyanide compounds, with alkaloids being the most abundant. The study found that there was a direct correlation between the concentration of the insecticide and the number of dead Aedes spp. larvae. The LC50 probit test revealed that pangi leaf extract belongs to the category of toxic substances. Several compounds, including alkaloids, flavonoids, tannins, saponins, and cyanide, may be responsible for the toxicity of pangi leaf extract. Thus, pangi leaf extract might be established as a means of overcoming numerous health issues caused by mosquito vectors.
{"title":"The potentials of Pangi leaf extract for Aedes spp. mosquito control","authors":"Yance Kaitana, N. Kurnia, M. Tulung, J. Tuda, J. Mamahit, T. Tallei","doi":"10.5455/jabet.2023.d112","DOIUrl":"https://doi.org/10.5455/jabet.2023.d112","url":null,"abstract":"Aedes spp. are the primary vectors of arboviruses such as dengue, Zika, and chikungunya. To date neither an approved vaccine nor a drug that can definitively prevent or treat these diseases, vector control continues to be an essential method of disease prevention. Plant-based insecticides are an alternative to chemical insecticides because they are less toxic to non-target insects and degrade more quickly. The Papuan people have used Pangi leaves for generations as a potent natural chemical against head lice and mosquito larvae. This study aimed to evaluate the potential of pangi (Pangium edule) leaf extract as a biolarvicide and mosquito repellent. Fresh pangi leaves were extracted with water by using a blender to pulverize the leaves. One-way ANOVA was used to examine the variance in mortality rates, and the LC50 value was calculated for probit analysis. Dead larvae were identified and counted. In addition to testing the effectiveness of the extracts as biolarvicide, the extract was also tested as potential insect repellents. The findings demonstrated that pangi leaf extract can kill mosquito larvae. The concentration of the extract affected larval mortality. Furthermore, the extract demonstrated its effectiveness as an insect repellent. Pangi leaf extract contains alkaloids, flavonoids, tannins, saponins, phenolic compounds, and cyanide compounds, with alkaloids being the most abundant. The study found that there was a direct correlation between the concentration of the insecticide and the number of dead Aedes spp. larvae. The LC50 probit test revealed that pangi leaf extract belongs to the category of toxic substances. Several compounds, including alkaloids, flavonoids, tannins, saponins, and cyanide, may be responsible for the toxicity of pangi leaf extract. Thus, pangi leaf extract might be established as a means of overcoming numerous health issues caused by mosquito vectors.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70796848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Supavinee Sukcharoen, Paphada Watcharapo, Kanokkorn Vichitsakul, Jiraporn Arunpanichlert, P. Rotkrua, B. Soontornworajit
Platelet-derived growth factor-BB (PDGF-BB), a multifunctional growth factor secreted by platelets, endothelial and cancer cells, functions as an efficient and accurate biomarker. PDGF- BB has been employed in various therapeutic applications. Tuning its bioactivity via a specific binding ligand could enhance the PDGF-BB functionality. Aptamer, a single-stranded oligonucleotide that exhibits a selective binding to PDGF-BB, is the molecule of our interest. This research aims to evaluate the PDGF-BB aptamer binding by growth factor-coated particles. PDGF-BB-coated particles were confirmed by ELISA, and aptamer binding evaluation was investigated by flow cytometry. To optimize the condition for binding evaluation, the washing cycle and concentration of PDGF-BB, polystyrene particle and aptamer were studied using flow cytometer. Based on the flow cytometry results, the concentration of PDGF-BB showed an optimal level on the number of fluorescent particles. The percentage of fluorescent particles increased when the washing cycle was reduced and the particle concentration was decreased. The percentage of fluorescent particles also increased proportionally with the aptamer concentration. Using a flow cytometer to detect fluorescent signal from growth factor-coated particle is a promising strategy to study biomolecular binding.
{"title":"Investigation of PDGF-BB aptamer binding using growth factor-coated particles and flow cytometry","authors":"Supavinee Sukcharoen, Paphada Watcharapo, Kanokkorn Vichitsakul, Jiraporn Arunpanichlert, P. Rotkrua, B. Soontornworajit","doi":"10.5455/jabet.2023.d122","DOIUrl":"https://doi.org/10.5455/jabet.2023.d122","url":null,"abstract":"Platelet-derived growth factor-BB (PDGF-BB), a multifunctional growth factor secreted by platelets, endothelial and cancer cells, functions as an efficient and accurate biomarker. PDGF- BB has been employed in various therapeutic applications. Tuning its bioactivity via a specific binding ligand could enhance the PDGF-BB functionality. Aptamer, a single-stranded oligonucleotide that exhibits a selective binding to PDGF-BB, is the molecule of our interest. This research aims to evaluate the PDGF-BB aptamer binding by growth factor-coated particles. PDGF-BB-coated particles were confirmed by ELISA, and aptamer binding evaluation was investigated by flow cytometry. To optimize the condition for binding evaluation, the washing cycle and concentration of PDGF-BB, polystyrene particle and aptamer were studied using flow cytometer. Based on the flow cytometry results, the concentration of PDGF-BB showed an optimal level on the number of fluorescent particles. The percentage of fluorescent particles increased when the washing cycle was reduced and the particle concentration was decreased. The percentage of fluorescent particles also increased proportionally with the aptamer concentration. Using a flow cytometer to detect fluorescent signal from growth factor-coated particle is a promising strategy to study biomolecular binding.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Gastric cancer (GC), which is the fourth most prevalent cancer in the world is significantly threatened the health of people, particularly those in developing nations. Nearly all significant pathological and physiological mechanisms, including apoptosis, proliferation, cell cycle, differentiation, as well as DNA damage, are regulated by miRNAs. This study looked at the miR-221-3p expression and to identified it's target genes in GC tissue specimens and cell lines, to comprehend the miR-221-3p influence in the development of GC. Methods: GC tissues and matched marginal tissues were taken from 50 patients undergo gastric surgery. MiR-221-3p mimics, inhibitors and negative controls (NC) were transfected into MKN-45 cells, using Lipofectamine RNAiMAX reagent. The proliferation was assessed by the MTT assay. Cell migration and invasion was assessed by Transwell assay. By combining Western blotting and qRT-PCR, the impact of miR-221-3p in the PIK3R1 expression in gastric cancer cells was examined. Results: Overexpression of miR-221-3p significantly enhanced the migration, invasion and proliferation of gastric cancer cells, conversely, transfection of miR-221-3p inhibitor led to opposite effect caused by overexpression of this miRNA on phenotypic characteristics of gastric cancer cell line. Additional investigation revealed that PIK3R1 was downregulated significantly by overexpression of miR-221-3p. Whereas, when the MKN-45 cells transfected with miR-221-3p inhibitor, PIK3R1 was noticeably overexpressed. Conclusions: Our current data indicate that miR-221-3p possibly work as a tumor promoter in the development of gastric cancer by negatively regulating PIK3R1 expression, hence miR-221-3p/ PIK3R1 highlighted as promising therapeutic targets or prognostic and diagnostic biomarkers for GC patients.
{"title":"MicroRNA-221-3p promotes cell proliferation, migration, and invasion in gastric cancer by modulating PIK3R1","authors":"","doi":"10.5455/jabet.2023.d133","DOIUrl":"https://doi.org/10.5455/jabet.2023.d133","url":null,"abstract":"Background: Gastric cancer (GC), which is the fourth most prevalent cancer in the world is significantly threatened the health of people, particularly those in developing nations. Nearly all significant pathological and physiological mechanisms, including apoptosis, proliferation, cell cycle, differentiation, as well as DNA damage, are regulated by miRNAs. This study looked at the miR-221-3p expression and to identified it's target genes in GC tissue specimens and cell lines, to comprehend the miR-221-3p influence in the development of GC. Methods: GC tissues and matched marginal tissues were taken from 50 patients undergo gastric surgery. MiR-221-3p mimics, inhibitors and negative controls (NC) were transfected into MKN-45 cells, using Lipofectamine RNAiMAX reagent. The proliferation was assessed by the MTT assay. Cell migration and invasion was assessed by Transwell assay. By combining Western blotting and qRT-PCR, the impact of miR-221-3p in the PIK3R1 expression in gastric cancer cells was examined. Results: Overexpression of miR-221-3p significantly enhanced the migration, invasion and proliferation of gastric cancer cells, conversely, transfection of miR-221-3p inhibitor led to opposite effect caused by overexpression of this miRNA on phenotypic characteristics of gastric cancer cell line. Additional investigation revealed that PIK3R1 was downregulated significantly by overexpression of miR-221-3p. Whereas, when the MKN-45 cells transfected with miR-221-3p inhibitor, PIK3R1 was noticeably overexpressed. Conclusions: Our current data indicate that miR-221-3p possibly work as a tumor promoter in the development of gastric cancer by negatively regulating PIK3R1 expression, hence miR-221-3p/ PIK3R1 highlighted as promising therapeutic targets or prognostic and diagnostic biomarkers for GC patients.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pangasius catfish (Pangasianodon hypophthalmus) is popularly cultured in Bangladesh but the farmers usually suffer financial losses due to their low market price. Development of new value-added products will enhance its popularity and acceptability to the consumers. This study was conducted to develop smoked products from pangasius catfish, to evaluate their quality and shelf-life at ambient (30-35°C) and refrigeration temperatures (4°C). Two treatments, dipped for 15 min in 10% (T1) and 15% (T2) salt solutions, and one untreated control were maintained. The smoking was conducted at 70-75°C in an improved traditional smoking kiln. The products were then sealed in polybags and stored separately at ambient and refrigeration temperatures. The sensory quality, proximate composition, salt content, total volatile base nitrogen (TVB-N) values, and viable bacterial counts were determined. The products stored at ambient temperature were rejected on day 5, 7 and 9; and those kept at refrigeration temperature, on day 28, 35, and 42, respectively for control, T1, and T2. Significant difference in proximate composition was observed between the treatments, storage conditions and periods. The TVB-N values were increased rapidly in control followed by the T1 samples, compare to T2 samples. Viable bacterial counts of the smoked products were gradually increased with the increasing storage periods. Considering all the assessed parameters, the T2 products were better in quality with longer shelf-life than those of control and T1. As consumers’ preference towards new fishery products has been changing, smoked products from pangasius catfish will be preferred for their taste, less bones, and higher shelf-life.
{"title":"Assessment of quality and shelf-life of salt-smoked cured products from Pangasius catfish at various temperatures","authors":"Srebash Saha, M. Haider, S. Chakraborty","doi":"10.5455/jabet.2023.d117","DOIUrl":"https://doi.org/10.5455/jabet.2023.d117","url":null,"abstract":"Pangasius catfish (Pangasianodon hypophthalmus) is popularly cultured in Bangladesh but the farmers usually suffer financial losses due to their low market price. Development of new value-added products will enhance its popularity and acceptability to the consumers. This study was conducted to develop smoked products from pangasius catfish, to evaluate their quality and shelf-life at ambient (30-35°C) and refrigeration temperatures (4°C). Two treatments, dipped for 15 min in 10% (T1) and 15% (T2) salt solutions, and one untreated control were maintained. The smoking was conducted at 70-75°C in an improved traditional smoking kiln. The products were then sealed in polybags and stored separately at ambient and refrigeration temperatures. The sensory quality, proximate composition, salt content, total volatile base nitrogen (TVB-N) values, and viable bacterial counts were determined. The products stored at ambient temperature were rejected on day 5, 7 and 9; and those kept at refrigeration temperature, on day 28, 35, and 42, respectively for control, T1, and T2. Significant difference in proximate composition was observed between the treatments, storage conditions and periods. The TVB-N values were increased rapidly in control followed by the T1 samples, compare to T2 samples. Viable bacterial counts of the smoked products were gradually increased with the increasing storage periods. Considering all the assessed parameters, the T2 products were better in quality with longer shelf-life than those of control and T1. As consumers’ preference towards new fishery products has been changing, smoked products from pangasius catfish will be preferred for their taste, less bones, and higher shelf-life.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Conventional remedies for management of Alzheimer’s disease (AD) and related cognitive deficits are not curative but relieves the symptoms and they cause adverse side effects. Alternatively, use of herbal therapies to manage cognitive illnesses has increased substantially. Vigna unguiculata is commonly utilized for nutritional benefits and management of neurological disorders in herbal medicine. The present study evaluated cognitive enhancing potential of V. unguiculata leaf aqueous extract in mice with ketamine-induced AD-like cognitive deficits. Cognitive performance indicated by step-through latency was assessed using passive avoidance test. Anti-acetylcholinesterase (anti-AChE) and antioxidant potential of the extract was assessed using brains of the test animals. Further, phytochemical constituents of the extracts were determined using LC-MS. Aqueous extract of V. unguiculata leaf demonstrated significant prowess to combat cognitive deficits in the test animals. This was evidenced by significantly higher (p < 0.001) step-through latencies in extract-treated mice than the untreated cognitively damaged mice. Moreover, cognitively damaged mice given the studied extract exhibited significantly less (p < 0.001) malondialdehyde levels and acetylcholinesterase activity than the negative control mice. This result confirmed antioxidant and anti-AChE properties of V. unguiculata, indicating its potential to attenuate oxidative stress in the brain and augment cholinergic transmission. Notably, some conventional therapies for cognitive disorders especially AD are AChE inhibitors. The studied extract contained phytocompounds such as flavonoids and phenolics with confirmed antioxidant and anti-AChE activities, thus, its cognitive enhancing efficacy could be attributed to these phytoconstituents. Collectively, this study upholds V. ungiculata usefulness in management of cognitive illnesses.
{"title":"Cognitive enhancing properties of aqueous leaf extract of Vigna unguiculata in ketamine-induced memory damage in mice","authors":"Daisy Kipkemoi, A. Ireri, M. Ngugi","doi":"10.5455/jabet.2023.d121","DOIUrl":"https://doi.org/10.5455/jabet.2023.d121","url":null,"abstract":"Conventional remedies for management of Alzheimer’s disease (AD) and related cognitive deficits are not curative but relieves the symptoms and they cause adverse side effects. Alternatively, use of herbal therapies to manage cognitive illnesses has increased substantially. Vigna unguiculata is commonly utilized for nutritional benefits and management of neurological disorders in herbal medicine. The present study evaluated cognitive enhancing potential of V. unguiculata leaf aqueous extract in mice with ketamine-induced AD-like cognitive deficits. Cognitive performance indicated by step-through latency was assessed using passive avoidance test. Anti-acetylcholinesterase (anti-AChE) and antioxidant potential of the extract was assessed using brains of the test animals. Further, phytochemical constituents of the extracts were determined using LC-MS. Aqueous extract of V. unguiculata leaf demonstrated significant prowess to combat cognitive deficits in the test animals. This was evidenced by significantly higher (p < 0.001) step-through latencies in extract-treated mice than the untreated cognitively damaged mice. Moreover, cognitively damaged mice given the studied extract exhibited significantly less (p < 0.001) malondialdehyde levels and acetylcholinesterase activity than the negative control mice. This result confirmed antioxidant and anti-AChE properties of V. unguiculata, indicating its potential to attenuate oxidative stress in the brain and augment cholinergic transmission. Notably, some conventional therapies for cognitive disorders especially AD are AChE inhibitors. The studied extract contained phytocompounds such as flavonoids and phenolics with confirmed antioxidant and anti-AChE activities, thus, its cognitive enhancing efficacy could be attributed to these phytoconstituents. Collectively, this study upholds V. ungiculata usefulness in management of cognitive illnesses.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetic kidney disease (DKD) is caused by a variety of processes. As a result, one biomarker is insufficient to represent the complete process. This study Evaluate the diagnostic value of serum kidney injury molecule-1(KIM-1) and cystatin C (CysC) as early biochemical markers of DKD and predictive their sensitivities and specificities as biomarkers of nephropathy in Iraqi type 2 diabetic (T2DM) patients. This cross-sectional study include 161 T2DM patients from Diabetes and Endocrinology Center at Merjan medical city in Babylon. Patients divided according to urinary albumin creatinine ratio(ACR) (Group1:ACR≤30mg/g,Group2:ACR>30mg/g). Random spot urine and fasting blood samples were taken from each patient and urinary ACR, blood glycated hemoglobin(HbA1c), and serum glucose, creatinine(SCr), lipid profile, CysC, KIM-1 were assayed, and the estimated glomerular filtration rat (eGFR) was calculated. When compared to the normoalbuminuric group, the DKD group had significantly greater prevalence of retinopathy, and significantly elevated HbA1c and total cholesterol values. Also had significantly greater serum levels of KIM-1 and CysC, and there is a significant (P-value< 0.01) positive correlation between them. In contrast, GFR was significantly higher in normoalbuminuric group and was significantly negatively correlated with both CysC and KIM-1. Multiple linear regression analysis, found that there were a significant positive association between CysC, KIM-1 and ACR. ROC analysis reveal that eGFR had the highest area under the curve(AUC=0.717), while SCr had the lowest AUC(0.556). In conclusion, Serum KIM-1 and CysC levels consider as early biomarker for DKD along with eGFR that consider the best diagnostic indicator of DKD, Additionally, there is a strong correlation between serum CysC and KIM-1 as well as other renal measures that indicate deteriorating kidney function.
{"title":"Kidney injury molecule-1 and cystatin C as early biomarkers for renal dysfunction in Iraqi type 2 diabetes mellitus patients","authors":"Ansam Yahya, Dheyaa Kadhim, Nassar Abdalhadi","doi":"10.5455/jabet.2023.d158","DOIUrl":"https://doi.org/10.5455/jabet.2023.d158","url":null,"abstract":"Diabetic kidney disease (DKD) is caused by a variety of processes. As a result, one biomarker is insufficient to represent the complete process. This study Evaluate the diagnostic value of serum kidney injury molecule-1(KIM-1) and cystatin C (CysC) as early biochemical markers of DKD and predictive their sensitivities and specificities as biomarkers of nephropathy in Iraqi type 2 diabetic (T2DM) patients. This cross-sectional study include 161 T2DM patients from Diabetes and Endocrinology Center at Merjan medical city in Babylon. Patients divided according to urinary albumin creatinine ratio(ACR) (Group1:ACR≤30mg/g,Group2:ACR>30mg/g). Random spot urine and fasting blood samples were taken from each patient and urinary ACR, blood glycated hemoglobin(HbA1c), and serum glucose, creatinine(SCr), lipid profile, CysC, KIM-1 were assayed, and the estimated glomerular filtration rat (eGFR) was calculated. When compared to the normoalbuminuric group, the DKD group had significantly greater prevalence of retinopathy, and significantly elevated HbA1c and total cholesterol values. Also had significantly greater serum levels of KIM-1 and CysC, and there is a significant (P-value< 0.01) positive correlation between them. In contrast, GFR was significantly higher in normoalbuminuric group and was significantly negatively correlated with both CysC and KIM-1. Multiple linear regression analysis, found that there were a significant positive association between CysC, KIM-1 and ACR. ROC analysis reveal that eGFR had the highest area under the curve(AUC=0.717), while SCr had the lowest AUC(0.556). In conclusion, Serum KIM-1 and CysC levels consider as early biomarker for DKD along with eGFR that consider the best diagnostic indicator of DKD, Additionally, there is a strong correlation between serum CysC and KIM-1 as well as other renal measures that indicate deteriorating kidney function.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70797715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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