HLA-B*0702 class-I allele, anti-FSH, anti-LH, and vitamin D3: Potential links with polycystic ovary syndrome in women of Erbil city, Iraq

Q3 Biochemistry, Genetics and Molecular Biology Journal of Advanced Biotechnology and Experimental Therapeutics Pub Date : 2023-01-01 DOI:10.5455/jabet.2023.d119
N. Berwary, Rand Aziz
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Abstract

Polycystic Ovary Syndrome (PCOS) is a widespread endocrine-reproductive-metabolic condition with severe implications for females’ health. The role of four essential parameters on PCOS, including Human Leukocyte Antigens (HLA) represented by the HLA-B*0702 allele, anti-Follicle Stimulating Hormone (anti-FSH) antibodies, anti-Luteinizing Hormone (anti-LH) antibodies, and vitamin D3 was investigated. A total of 100 samples were collected from Kurdish women attended the Maternity Teaching Hospital and some private hospitals/laboratories in Erbil City from October, 2021 to January, 2022. The samples were genotyped using a PCR-based technique with specific sequence primers. The levels of anti-FSH and anti-LH antibodies were determined using an Enzyme-Linked Immunosorbent Assay (ELISA), while vitamin D3 levels were measured by an Electrochemiluminescence (ECL) test on Cobas e411 immunoassay system. The Odds Ratio (OR) of 2.167 at a 95% Confidence Interval (CI) of 0.8167 to 6.330, indicated an essential link between the HLA-B*0702 allele and a risk of PCOS. Anti-FSH and anti-LH antibodies were significantly greater in PCOS patients, notably infertile women, than in healthy controls. A significant positive linear correlation was observed between antibodies against FSH and LH in patients. Most patients and controls had hypovitaminosis D3, with a significant difference (P-value  0.01). The results indicated that the HLA-B*0702 allele is associated with PCOS susceptibility and could be used as an immunogenetic marker. It also supported the idea that anti-FSH and anti-LH antibodies are naturally presence antibodies in PCOS patients instead of signs for autoimmunity. Women suffer from PCOS are also more prone to develop vitamin D3 deficiency.
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HLA-B*0702 i类等位基因、抗fsh、抗lh和维生素D3:与伊拉克埃尔比勒市妇女多囊卵巢综合征的潜在联系
多囊卵巢综合征(PCOS)是一种广泛存在的内分泌生殖代谢疾病,严重影响女性健康。研究以HLA- b *0702等位基因为代表的人类白细胞抗原(HLA)、抗促卵泡激素(fsh)抗体、抗黄体生成素(lh)抗体和维生素D3在PCOS发病中的作用。从2021年10月至2022年1月在埃尔比勒市产科教学医院和一些私立医院/实验室就诊的库尔德妇女中共收集了100个样本。使用基于pcr的技术和特定序列引物对样品进行基因分型。采用酶联免疫吸附法(ELISA)测定抗fsh和抗lh抗体水平,采用Cobas e411免疫分析系统电化学发光法(ECL)测定维生素D3水平。比值比(OR)为2.167,95%可信区间(CI)为0.8167 ~ 6.330,表明HLA-B*0702等位基因与PCOS风险之间存在重要联系。PCOS患者的抗fsh和抗lh抗体明显高于健康对照组,尤其是不孕妇女。患者FSH抗体与LH抗体呈显著的线性正相关。多数患者与对照组存在维生素D3缺乏症,差异有统计学意义(p值为0.01)。结果表明,HLA-B*0702等位基因与PCOS易感性相关,可作为免疫遗传标记。这也支持了抗卵泡刺激素和抗黄体生成素抗体是多囊卵巢综合征患者体内天然存在的抗体,而不是自身免疫症状的观点。患有多囊卵巢综合征的女性也更容易缺乏维生素D3。
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来源期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
Journal of Advanced Biotechnology and Experimental Therapeutics Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.90
自引率
0.00%
发文量
41
审稿时长
8 weeks
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