Larvicidal Activity of Tinospora crispa (Menispermaceae) Fruit Extract Against Filarial Vector Culex quinquefasciatus

Abstract

Control of filarial vector; Culex quinquefasciatus larvae using target specific, non-resistant and eco-friendly phytochemicals are an alternative way to prevent the spread of this vector-borne disease. This study was carried out to assess the mosquito larvicidal potency of Tinospora crispa fruit extract against Cx. quinquefasciatus . Crude and petroleum ether extracts were examined against all larval instars. LC 50 & LC 90 values were determined through log-probit analysis. The lowest value was obtained at 72 hours of exposure in both crude and petroleum ether extracts. Regression and ANOVA analysis were also done for statistical judgement. A preliminary phytochemical analysis of the crude extract of fruits was also done to know the possible active ingredients which are believed to be responsible for larval toxicity. The analyses revealed the presence of tannin and steroid. No abnormality was seen in the motion and behaviour of non- target organisms when they were exposed to LC 50 values of crude and solvent extracts. In conclusion, T. crispa fruit extract has the potency to fight effectively against Cx. quinquefasciatus larvae.