Growth Rate Modulation Enables Coexistence in a Competitive Exclusion Scenario Between Microbial Eukaryotes

Abstract

Coexistence usually are exceeding the explicable rate by competitive exclusion principle. Since the pioneer Gause, many studies have used protist microcosm systems to study competitive exclusion. We explored a two-species system with the testate-amoebae: (Arcella intermedia and Pyxidicula operculata), where competitive exclusion is expected to occur. We determined their growth curves individually and under competitive interaction. We used a state-space model to represent system dynamics and calculated posterior population sizes simulating competition dynamics. Contrarily to our expectation, Arcella and Pyxidicula showed similar growth rates (1.37 and 1.46 days–1 respectively) and only different carrying capacity (1,997 and 25,108 cells cm–2 respectively). The maximum number of cells of both species when growing in competition was much lower if compared to the monospecific cultures (in average, 73% and 80% less for Arcella and Pyxidicula respectively). However, our competition experiments always resulted in coexistence. According to the models, the drop in growth rates and stochasticity mainly explains our coexistence results. We propose that a context of ephemeral resources can explain these results. Additionally, we propose generating factors of stochasticity as intraspecific variation, small population effects, toxicity of waste products and influence of the bacterial community.