PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT ACTIVITY AND FTIR SPECTROSCOPIC ANALYSIS OF RED LEAF LETTUCE AND GREEN LEAF LETTUCE (LACTUCA SATIVA L.)

Q4 Pharmacology, Toxicology and Pharmaceutics INDIAN DRUGS Pub Date : 2023-05-28 DOI:10.53879/id.60.05.13378
N. Utami, Prashinta N. Damayanti
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Abstract

This study was conducted to analyze the phytochemical compounds, the profile of infrared spectrophotometric, total phenolic contents (TPC) and antioxidant activity of ethanol extract of red leaf lettuce (RL) and green leaf lettuce (GL). RL and GL were extracted with 70 % ethanol using the maceration method for 3 days and re-maceration for 1 day. The secondary metabolites in ethanol extracts were evaluated by phytochemical analysis and profile spectra infrared. Estimation of TPC was conducted by the FolinCiocalteu methods. The antioxidant activity assay was conducted by ABTS and DPPH methods. The determination of TPC showed that the ethanol extract of RL was higher than the ethanol extract of GL. RL has stronger antioxidant activity than GL. The presence of hydroxyl group in the phenolics directly correlates with the antioxidant activity, so consumers could use them as natural antioxidants or to functionalize foods. This understanding is important for improving the safety and quality of leaf lettuce.
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红叶莴苣和绿叶莴苣的植物化学分析、抗氧化活性及红外光谱分析
对红叶莴苣(RL)和绿叶莴苣(GL)乙醇提取物的植物化学成分、红外光谱、总酚含量(TPC)和抗氧化活性进行了研究。RL和GL用70%乙醇浸提3 d,再浸提1 d。利用植物化学分析和红外光谱对乙醇提取物的次生代谢产物进行了评价。采用folincocalteu方法对TPC进行估计。采用ABTS法和DPPH法测定其抗氧化活性。TPC测定结果表明,牛蒡子醇提物的TPC含量高于牛蒡子醇提物,其抗氧化活性强于牛蒡子醇提物,其酚类物质中羟基的存在与抗氧化活性直接相关,可作为天然抗氧化剂或功能化食品。这对提高叶莴苣的安全和质量具有重要意义。
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来源期刊
INDIAN DRUGS
INDIAN DRUGS Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
0.30
自引率
0.00%
发文量
98
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