Liver regeneration after hepatectomy is significantly suppressed in a muscular atrophy mouse model

IF 3.2 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Journal of Hepato‐Biliary‐Pancreatic Sciences Pub Date : 2023-11-01 DOI:10.1002/jhbp.1386

Kei Hagiwara, Akira Watanabe, Norifumi Harimoto, Kenichiro Araki, Takehiko Yokobori, Ryo Muranushi, Kouki Hoshino, Norihiro Ishii, Mariko Tsukagoshi, Ken Shirabe

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Abstract

Background

Sarcopenia is a syndrome characterized by progressive and generalized loss of skeletal muscle mass and strength. As reported in previous studies, the loss of skeletal muscle mass is associated with poor liver regeneration after hepatectomy. It is considered important to clarify the effect of sarcopenia on liver regeneration; however, there are no reports about model animals for sarcopenia. We focused on the peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1α) transgenic mice that overexpressed PGC-1α, specifically for skeletal muscle, and showed significant atrophy of type 2B fiber-rich muscles like sarcopenia.

Methods

We performed 70% hepatectomy using PGC-1α transgenic mice and examined the liver regeneration rate and the effects of branched-chain amino acids (BCAA) after hepatectomy.

Results

Liver regeneration after 70% hepatectomy was significantly suppressed in the PGC-1α transgenic mice. In addition, a decrease in the blood BCAA concentration and a decrease in the liver glycogen content after 70% hepatectomy were observed in the PGC-1α transgenic mice. By administering BCAA before and after surgery, it was clarified that a significant increase in the blood BCAA concentration was observed and the liver regeneration rate was improved in the PGC-1α transgenic mice.

Conclusions

BCAA administration may improve the suppression of liver regeneration in patients with sarcopenia.

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肝切除术后的肝脏再生在肌肉萎缩小鼠模型中受到显著抑制。

背景：Sarcopenia是一种以骨骼肌质量和力量进行性和全身性丧失为特征的综合征。正如先前的研究所报道的，骨骼肌质量的损失与肝切除术后肝脏再生不良有关。阐明少肌症对肝脏再生的影响被认为是重要的；然而，目前还没有关于少肌症模型动物的报道。我们重点研究了过氧化物酶体增殖物激活受体γ共激活因子-1α（PGC-1α）转基因小鼠，该小鼠过表达PGC-1 a，特别是对骨骼肌，并表现出2B型富含纤维的肌肉的显著萎缩，如少肌症。方法：用PGC-1α转基因小鼠进行70%肝切除术，观察肝切除后肝再生率和支链氨基酸（BCAA）的影响。结果：PGC-1α转基因小鼠70%肝切除后的肝脏再生受到显著抑制。此外，在PGC-1α转基因小鼠中观察到70%肝切除后血液BCAA浓度降低和肝糖原含量降低。通过在手术前后给予BCAA，可以清楚地观察到PGC-1α转基因小鼠的血液BCAA浓度显著增加，并且肝脏再生率提高。结论：BCAA给药可改善少肌症患者对肝再生的抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Hepato‐Biliary‐Pancreatic Sciences GASTROENTEROLOGY & HEPATOLOGY-SURGERY

自引率

10.00%

发文量

178

审稿时长

6-12 weeks

期刊介绍： The Journal of Hepato-Biliary-Pancreatic Sciences (JHBPS) is the leading peer-reviewed journal in the field of hepato-biliary-pancreatic sciences. JHBPS publishes articles dealing with clinical research as well as translational research on all aspects of this field. Coverage includes Original Article, Review Article, Images of Interest, Rapid Communication and an announcement section. Letters to the Editor and comments on the journal’s policies or content are also included. JHBPS welcomes submissions from surgeons, physicians, endoscopists, radiologists, oncologists, and pathologists.