Suppression of subgingival bacteria by antimicrobial photodynamic therapy using transgingival irradiation: A randomized clinical trial

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontology Pub Date : 2023-11-01 DOI:10.1002/JPER.23-0328
Jun-ichiro Hayashi, Kohta Ono, Yuki Iwamura, Yasuyuki Sasaki, Tasuku Ohno, Ryoma Goto, Eisaku Nishida, Genta Yamamoto, Takeshi Kikuchi, Naoya Higuchi, Akio Mitani, Mitsuo Fukuda
{"title":"Suppression of subgingival bacteria by antimicrobial photodynamic therapy using transgingival irradiation: A randomized clinical trial","authors":"Jun-ichiro Hayashi,&nbsp;Kohta Ono,&nbsp;Yuki Iwamura,&nbsp;Yasuyuki Sasaki,&nbsp;Tasuku Ohno,&nbsp;Ryoma Goto,&nbsp;Eisaku Nishida,&nbsp;Genta Yamamoto,&nbsp;Takeshi Kikuchi,&nbsp;Naoya Higuchi,&nbsp;Akio Mitani,&nbsp;Mitsuo Fukuda","doi":"10.1002/JPER.23-0328","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Antimicrobial photodynamic therapy (aPDT) is an effective method for eradicating bacteria in periodontal therapy. Standard aPDT requires the insertion of a laser tip into a periodontal pocket, in which the direction of irradiation is limited. Therefore, we devised an aPDT method that uses a transgingival near-infrared wavelength and indocyanine green-encapsulated and chitosan-coated nanoparticles as a photosensitizer.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Forty patients undergoing supportive periodontal therapy, who had a single root tooth with a pocket of 5 mm or deeper, were used as subjects. In the test group, aPDT was performed by laser irradiation from outside the gingiva using photosensitizer nanoparticles. In the control group, pseudo aPDT without photosensitizer was performed by transgingival irradiation. Subgingival plaque was sampled from inside the pocket before, immediately after, and 1 week after treatment, and evaluated by colony counting and real-time polymerase chain reaction.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>There were no significant differences in age, sex, periodontal pocket depth, and bleeding on probing between the test and control groups. Compared with the colony count before treatment, the count in the test group was significantly reduced immediately after treatment. The number of patients with colony reduction to ≤50% and ≤10% was significantly higher in the test group than in the control group. None of the participants reported pain, although one participant reported discomfort.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>As a bacterial control method for residual pockets in patients undergoing supportive periodontal therapy, transgingival aPDT is a promising treatment strategy that is not generally accompanied by pain or discomfort.</p>\n </section>\n </div>","PeriodicalId":16716,"journal":{"name":"Journal of periodontology","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/JPER.23-0328","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of periodontology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/JPER.23-0328","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0

Abstract

Background

Antimicrobial photodynamic therapy (aPDT) is an effective method for eradicating bacteria in periodontal therapy. Standard aPDT requires the insertion of a laser tip into a periodontal pocket, in which the direction of irradiation is limited. Therefore, we devised an aPDT method that uses a transgingival near-infrared wavelength and indocyanine green-encapsulated and chitosan-coated nanoparticles as a photosensitizer.

Methods

Forty patients undergoing supportive periodontal therapy, who had a single root tooth with a pocket of 5 mm or deeper, were used as subjects. In the test group, aPDT was performed by laser irradiation from outside the gingiva using photosensitizer nanoparticles. In the control group, pseudo aPDT without photosensitizer was performed by transgingival irradiation. Subgingival plaque was sampled from inside the pocket before, immediately after, and 1 week after treatment, and evaluated by colony counting and real-time polymerase chain reaction.

Results

There were no significant differences in age, sex, periodontal pocket depth, and bleeding on probing between the test and control groups. Compared with the colony count before treatment, the count in the test group was significantly reduced immediately after treatment. The number of patients with colony reduction to ≤50% and ≤10% was significantly higher in the test group than in the control group. None of the participants reported pain, although one participant reported discomfort.

Conclusion

As a bacterial control method for residual pockets in patients undergoing supportive periodontal therapy, transgingival aPDT is a promising treatment strategy that is not generally accompanied by pain or discomfort.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
抗微生物光动力疗法经牙龈照射抑制龈下细菌:一项随机临床试验。
背景:抗菌光动力疗法(aPDT)是牙周治疗中一种有效的除菌方法。标准aPDT需要将激光尖端插入牙周袋中,其中照射方向受到限制。因此,我们设计了一种aPDT方法,该方法使用跨牙龈近红外波长和吲哚菁绿包裹和壳聚糖包裹的纳米颗粒作为光敏剂。方法:选择40名接受支持性牙周治疗的患者作为受试者,他们的单根牙的窝深为5毫米或5毫米。在试验组中,通过使用光敏剂纳米颗粒从牙龈外进行激光照射来进行aPDT。在对照组中,通过经牙龈照射进行不含光敏剂的伪aPDT。在治疗前、治疗后立即和治疗后1周从袋内取样龈下菌斑,并通过菌落计数和实时聚合酶链式反应进行评估。结果:试验组和对照组在年龄、性别、牙周袋深度和探查出血方面没有显著差异。与治疗前的菌落计数相比,试验组的菌落计数在治疗后立即显著减少。试验组菌落减少至≤50%和≤10%的患者数量显著高于对照组。没有一名参与者报告疼痛,但有一名参与者表示不适。结论:作为一种治疗支持性牙周治疗患者残留菌袋的细菌控制方法,经牙龈aPDT是一种很有前途的治疗策略,通常不会伴随疼痛或不适。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
期刊最新文献
Appraising the life-course impact of Epstein-Barr virus exposure and its genetic signature on periodontitis. Effectiveness of nonsurgical re‐instrumentation: Tooth‐related factors ISSUE INFORMATION Gingival inflammation and leukocyte-endothelium cell interactions in women with polycystic ovary syndrome. Clinical outcomes of using operating microscope for alveolar ridge preservation: A randomized controlled trial.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1