Expression of m6A Regulator Genes can Facilitate the Diagnosis of Chronic Heart Failure.

IF 0.7 4区 医学 Q4 CARDIAC & CARDIOVASCULAR SYSTEMS Heart Surgery Forum Pub Date : 2023-10-11 DOI:10.59958/hsf.6335
Fang Zhou, Yang Yu, Yukun Li, Jessica Chen, Songnan Wen, Nian Liu, Xin Li, Rong Bai, Wanyao Yan
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Abstract

Background: RNA N6-methyladenosine (m6A) is the most common type of modification in eukaryotic mRNA. The relationship between m6A modification and disease has been studied extensively, but there have been few studies on chronic heart failure (CHF). This study investigated a possible role for m6A in the diagnosis of CHF.

Methods: Seven candidate m6A regulators (writers: WTAP and ZC3H13; readers: YTHDF3, FMR1, IGFBP1, and ELAVL1; eraser: FTO) were identified using a random forest (RF) model and the GSE5406 dataset from the Gene Expression Omnibus database. A nomogram model was developed to predict the risk of CHF, while consensus clustering methodology assigned CHF samples into two m6A patterns (cluster A and cluster B) according to the 7 candidate m6A regulators. Principal component analysis was used to calculate an m6A score for each sample and to quantify m6A patterns.

Results: Decision curve analysis and the nomogram model were used to obtain predictions that may be of clinical use. Patients in cluster B had higher m6A scores than patients in cluster A. Cluster B patients also had higher expression levels (ELs) of IL-4, IL-5, IL-10 and IL-13 than patients in cluster A, whereas cluster A patients had a higher EL for IL-33. The m6A cluster B pattern likely represents the ischemic heart failure (HF) disease group.

Conclusion: m6A regulators are important in the pathogenesis of CHF associated with ischemic and idiopathic dilated cardiomyopathy, and may prove useful for the diagnosis and treatment of CHF.

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m6A调节基因的表达可促进慢性心力衰竭的诊断。
背景:核糖核酸N6-甲基腺苷(m6A)是真核细胞信使核糖核酸中最常见的修饰类型。m6A修饰与疾病之间的关系已被广泛研究,但对慢性心力衰竭(CHF)的研究很少。本研究调查了m6A在CHF诊断中的可能作用。方法:使用随机森林(RF)模型和基因表达综合数据库中的GSE5406数据集鉴定了七种候选m6A调节因子(作者:WTAP和ZC3H13;读者:YTHDF3、FMR1、IGFBP1和ELAVL1;擦除器:FTO)。开发了一个列线图模型来预测CHF的风险,而共识聚类方法根据7个候选m6A调节因子将CHF样本分配为两个m6A模式(聚类A和聚类B)。主成分分析用于计算每个样本的m6A得分,并量化m6A模式。结果:决策曲线分析和列线图模型用于获得可能具有临床应用价值的预测。集群B中的患者比集群A中的患者具有更高的m6A评分。集群B中患者的IL-4、IL-5、IL-10和IL-13的表达水平(EL)也比集群A的患者更高,而集群A中患者的IL-13的EL更高。m6A簇B模式可能代表缺血性心力衰竭(HF)疾病组。结论:m6A调节因子在CHF合并缺血性和特发性扩张型心肌病的发病机制中具有重要作用,可用于CHF的诊断和治疗。
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来源期刊
Heart Surgery Forum
Heart Surgery Forum 医学-外科
CiteScore
1.20
自引率
16.70%
发文量
130
审稿时长
6-12 weeks
期刊介绍: The Heart Surgery Forum® is an international peer-reviewed, open access journal seeking original investigative and clinical work on any subject germane to the science or practice of modern cardiac care. The HSF publishes original scientific reports, collective reviews, case reports, editorials, and letters to the editor. New manuscripts are reviewed by reviewers for originality, content, relevancy and adherence to scientific principles in a double-blind process. The HSF features a streamlined submission and peer review process with an anticipated completion time of 30 to 60 days from the date of receipt of the original manuscript. Authors are encouraged to submit full color images and video that will be included in the web version of the journal at no charge.
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