Biomechanical Scaffolds of Decellularized Heart Valves Modified by Electrospun Polylactic Acid

IF 3.1 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Applied Biochemistry and Biotechnology Pub Date : 2023-11-03 DOI:10.1007/s12010-023-04756-8

Chaorong Wang, Qingqing Chen, Han Wang, Hanlin Gang, Yingshan Zhou, Shaojin Gu, Ruoyun Zhang, Weilin Xu, Hongjun Yang

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Abstract

Enhancing the mechanical properties and cytocompatibility of decellularized heart valves is the key to promote the application of biological heart valves. In order to further improve the mechanical properties, the electrospinning and non-woven processing methods are combined to prepare the polylactic acid (PLA)/decellularized heart valve nanofiber-reinforced sandwich structure electrospun scaffold. The effect of electrospinning time on the performance of decellularized heart valve is investigated from the aspects of morphology, mechanical properties, softness, and biocompatibility of decellularized heart valve. Results of the mechanical tests show that compared with the pure decellularized heart valve, the mechanical properties of the composite heart valve were significantly improved with the tensile strength increasing by 108% and tensile strain increased by 571% when the electrospinning time exceeded 2 h. In addition, with this electrospinning time, the composite heart valve has a certain promoting effect on the human umbilical vein endothelial cells proliferation behavior. This work provides a promising foundation for tissue heart valve reendothelialization to lay the groundwork for organoid.

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电纺聚乳酸修饰脱细胞心脏瓣膜的生物力学支架。

提高脱细胞心脏瓣膜的力学性能和细胞相容性是促进生物心脏瓣膜应用的关键。为了进一步提高力学性能，将静电纺丝和非织造处理方法相结合，制备了聚乳酸/脱细胞心脏瓣膜纳米纤维增强夹层结构静电纺丝支架。从脱细胞心脏瓣膜的形态、力学性能、柔软度和生物相容性等方面研究了静电纺丝时间对脱细胞心脏瓣性能的影响。力学测试结果表明，与纯脱细胞心脏瓣膜相比，当静电纺丝时间超过2小时时，复合心脏瓣膜的力学性能显著改善，拉伸强度增加了108%，拉伸应变增加了571%。此外，随着静电纺丝时间的延长，复合心脏瓣膜对人脐静脉内皮细胞的增殖行为有一定的促进作用。这项工作为组织心脏瓣膜再内皮化提供了一个有希望的基础，为类器官奠定了基础。

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来源期刊

Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学

CiteScore

5.70

自引率

6.70%

发文量

460

审稿时长

5.3 months

期刊介绍： This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.