Transformation of Mercuric Ions to Mercury Nanoparticles in Diatom Chaetoceros curvisetus

Abstract

Particulate HgS play crucial roles in the mercury (Hg) cycle. Approximately 20–90% of dissolved Hg can be transformed into particulate HgS by algae. However, detailed knowledge regarding these particles, including sizes and distribution, remains unknown. The present study explored the formation, distribution, and excretion of mercury nanoparticles (HgNPs) in diatom Chaetoceros curvisetus. The results demonstrated that HgNPs (HgS nanoparticles, 29.6–66.2 nm) formed intracellularly upon exposure to 5.0–100.0 μg L^–1 Hg(II), accounting for 12–27% of the total Hg. HgNP concentrations significantly increased with increasing intracellular Hg(II) concentrations, while their sizes remained unaffected. HgNPs formed intracellularly and partly accumulated inside the cells (7–11%). Subsequently, the sizes of intracellular HgNPs gradually decreased to facilitate expulsion, 21–50% of which were excreted. These suggested the vital roles of HgNPs in comprehending marine Hg fate. Their unique physicochemical properties and bioavailability would influence Hg biotransformation in the ocean. Additionally, both intracellular and extracellular HgNPs contributed to Hg settling with cells, ultimately leading to Hg burial in sediments. Overall, these findings further deepened our understanding of Hg biotransformation and posed challenges in accurately estimating marine Hg flux and Hg burial.