Antimicrobial and antibiofilm activity of fungal metabolites on methicillin-resistant Staphylococcus aureus (ATCC 43300) mediated by SarA and AgrA.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-08-01 Epub Date: 2023-11-20 DOI:10.1080/08927014.2023.2276926

Oswaldo Pablo Martínez-Rodríguez, Rodolfo García-Contreras, Rodrigo Aguayo-Ortiz, Mario Figueroa

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) increases its antibiotic resistance by forming biofilms. Natural products (NP) or specialized metabolites have demonstrated their ability to decrease the virulence and pathogenesis of MRSA infections by inhibiting biofilm formation. The present study evaluated the antimicrobial and antibiofilm potential against MRSA of a small library of fungal NP isolated from Mexican biodiversity. The most potent antibacterial activity was observed for myrotecisin B, epiequisetin, equisetin, stachybotrolide acetate, monorden A, zearalenone, fuscin, and fusarubin. On the other hand, epifiscalin C, fiscalin C, dimethylglyotoxin, aspernolide B, and butyrolactones I and IV inhibited the biofilm formation without decreasing bacterial growth. To determine the putative mechanism of action of these compounds, docking analyses were performed against SarA and AgrA proteins, targets known to regulate biofilm production in MRSA. Overall, the results demonstrate that fungal NP may act as potential antibiofilm agents for treating MRSA infections.

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SarA和AgrA介导的真菌代谢产物对耐甲氧西林金黄色葡萄球菌（ATCC 43300）的抗菌和抗菌膜活性。

耐甲氧西林金黄色葡萄球菌（MRSA）通过形成生物膜来增加其抗生素耐药性。天然产物（NP）或特殊代谢产物已证明其通过抑制生物膜形成来降低MRSA感染的毒力和发病机制的能力。本研究评估了从墨西哥生物多样性中分离的一个小型真菌NP文库对MRSA的抗菌和抗生物膜潜力。观察到最有效的抗菌活性是myrotecin B、表木犀草素、木犀草碱、乙酸斯塔希罗利、单烯菌素A、玉米赤霉烯酮、褐素和镰刀菌素。另一方面，表菲沙林C、菲沙林C、二甲基glyotoxin、aspenolide B和丁内酯I和IV抑制生物膜的形成，而不降低细菌生长。为了确定这些化合物的假定作用机制，对已知调节MRSA中生物膜产生的靶点SarA和AgrA蛋白进行了对接分析。总之，结果表明真菌NP可能作为治疗MRSA感染的潜在抗生物膜剂。

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