Diuretic, Natriuretic, And Ca2+-Sparing Effect Of The Alkaloid Boldine In Rats.

IF 1.7 Q3 PHARMACOLOGY & PHARMACY Drug Research Pub Date : 2023-11-01 Epub Date: 2023-11-07 DOI:10.1055/a-2182-3665
Viviane Miranda Bispo Steimbach, Ritade Cássia Vilhena da Silva, Luísa Nathália Bolda Mariano, Mariana Zanovello, Anelise Felício Macarini, Luisa Mota da Silva, Priscila de Souza
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Abstract

Background: Previous studies indicate the renal vasodilating effects of boldine, an alkaloid found in Peumus boldus. However, its potential to induce diuresis still needs to be studied.

Methods: Wistar rats were used and the urine volume was noted for 8 h and further studied.

Results: The acute treatment at 0.1 and 0.3 mg/kg of boldine showed a diuretic, natriuretic, and Ca2+-sparing effect in rats without changing the urinary elimination of K+and Cl-. When boldine was given in combination with hydrochlorothiazide, there was an increase in urinary volume compared to the vehicle group. However, this was not different from the treatments in its isolated form. Urine Ca2+values ​​remained low but were not enhanced by this association. The excretion of Na+and Cl- was significantly increased compared to the group that received only vehicle or boldine. On the other hand, although the association of amiloride plus boldine did not result in a diuretic effect, the increase in Na+and the reduction in K+excretion were significantly potentiated. Furthermore, in the presence of the non-selective muscarinic receptor antagonist atropine, boldine showed reduced capacity to increase urinary volume, maintaining the natriuretic and Ca2+-sparing effect, besides a very evident K+-sparing action. Similar results were obtained in the presence of the non-selective cyclooxygenase inhibitor indomethacin. Furthermore, boldine showed an ex vivo antiurolithiasis activity, reducing calcium oxalate's precipitation and crystallization.

Conclusions: This study reveals the diuretic, natriuretic, Ca2+-sparing, and antiurolithiatic effects of boldine, an action possibly related to muscarinic receptor activation and prostanoid generation.

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生物碱Boldine对大鼠的利尿、利钠素和Ca2+的抑制作用。
背景:先前的研究表明,在白头翁中发现的生物碱博尔丁具有肾血管舒张作用。然而,其诱导利尿的潜力仍有待研究。方法:采用Wistar大鼠,记录8只大鼠的尿量 h,并进一步研究。结果:急性治疗在0.1和0.3 mg/kg的博丁在大鼠中显示出利尿、利钠素和Ca2+保留作用,而不改变尿中K+和Cl-的清除。当博丁与氢氯噻嗪联合用药时,与溶媒组相比,尿量增加。然而,这与分离形式的治疗没有什么不同​​仍然很低,但没有因这种关联而增强。与仅接受赋形剂或boldine的组相比,Na+和Cl-的排泄显著增加。另一方面,尽管阿米洛利和博丁的联合作用没有产生利尿作用,但Na+的增加和K+排泄的减少显著增强。此外,在非选择性毒蕈碱受体拮抗剂阿托品存在的情况下,除了非常明显的K+保留作用外,boldine显示出增加尿量的能力降低,维持了钠尿和Ca2+保留作用。在非选择性环氧合酶抑制剂吲哚美辛的存在下也获得了类似的结果。此外,boldine具有体外抗尿石活性,减少草酸钙的沉淀和结晶。结论:本研究揭示了博丁的利尿、利钠素、Ca2+保留和抗尿锂作用,其作用可能与毒蕈碱受体激活和前列腺素生成有关。
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来源期刊
Drug Research
Drug Research PHARMACOLOGY & PHARMACY-
CiteScore
3.50
自引率
0.00%
发文量
67
期刊介绍: Drug Research (formerly Arzneimittelforschung) is an international peer-reviewed journal with expedited processing times presenting the very latest research results related to novel and established drug molecules and the evaluation of new drug development. A key focus of the publication is translational medicine and the application of biological discoveries in the development of drugs for use in the clinical environment. Articles and experimental data from across the field of drug research address not only the issue of drug discovery, but also the mathematical and statistical methods for evaluating results from industrial investigations and clinical trials. Publishing twelve times a year, Drug Research includes original research articles as well as reviews, commentaries and short communications in the following areas: analytics applied to clinical trials chemistry and biochemistry clinical and experimental pharmacology drug interactions efficacy testing pharmacodynamics pharmacokinetics teratology toxicology.
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