Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction.

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Animal Science and Biotechnology Pub Date : 2023-11-06 DOI:10.1186/s40104-023-00938-w
Estel Viñolas-Vergés, Jordi Ribas-Maynou, Isabel Barranco, Camila Peres Rubio, Sergi Bonet, Jordi Roca, Marc Yeste
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Abstract

Background: Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo development. In some mammals, like pigs, ejaculates are emitted in three separate fractions: pre-sperm, sperm-rich (SRF) and post sperm-rich (PSRF). These fractions are known to vary in volume, sperm concentration and quality, as well as in the origin and composition of seminal plasma (SP), with differences being also observed within the SRF one. Yet, whether disparities in the DNA integrity and chromatin condensation and protamination of their sperm exist has not been interrogated.

Results: This study determined chromatin protamination (Chromomycin A3 test, CMA3), condensation (Dibromobimane test, DBB), and DNA integrity (Comet assay) in the pig sperm contained in the first 10 mL of the SRF (SRF-P1), the remaining portion of the sperm-rich fraction (SRF-P2), and the post sperm-rich fraction (PSRF). While chromatin protamination was found to be similar between the different ejaculate fractions (P > 0.05), chromatin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF (P = 0.018 and P = 0.004, respectively). Regarding DNA integrity, no differences between fractions were observed (P > 0.05). As the SRF-P1 has the highest sperm concentration and ejaculate fractions are known to differ in antioxidant composition, the oxidative stress index (OSi) in SP, calculated as total oxidant activity divided by total antioxidant capacity, was tested and confirmed to be higher in the SRF-P1 than in SRF-P2 and PSRF (0.42 ± 0.06 vs. 0.23 ± 0.09 and 0.08 ± 0.00, respectively; P < 0.01); this index, in addition, was observed to be correlated to the sperm concentration of each fraction (Rs = 0.973; P < 0.001).

Conclusion: While sperm DNA integrity was not found to differ between ejaculate fractions, SRF-P1 and SRF-P2 were observed to exhibit greater chromatin condensation than the PSRF. This could be related to the OSi of each fraction.

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猪精子的染色质凝聚而非DNA完整性在富含精子的部分中更大。
背景:精子染色质的前胺化和浓缩以及DNA的完整性在受精和胚胎发育过程中起着至关重要的作用。在一些哺乳动物中,如猪,精液分为三个不同的部分:精子前、富含精子(SRF)和富含精子后(PSRF)。众所周知,这些组分在体积、精子浓度和质量以及精浆(SP)的来源和组成方面都有所不同,SRF组分也存在差异。然而,他们精子的DNA完整性、染色质浓缩和精蛋白鉴定是否存在差异还没有被质疑。结果:本研究测定了前10mL SRF(SRF-P1)、富精组分(SRF-P2)和精后组分(PSRF)中猪精子的染色质精蛋白(色霉素A3试验,CMA3)、缩合(二溴比曼试验,DBB)和DNA完整性(彗星试验)。染色质精蛋白作用在不同精液组分间相似(P > 0.05),SRF-P1和SRF-P2的染色质浓缩程度高于PSRF(P = 0.018和P = 0.004)。在DNA完整性方面,组分之间没有观察到差异(P > 0.05)。由于SRF-P1具有最高的精子浓度,并且已知射精部分的抗氧化成分不同,因此测试并证实SRF-P1中的氧化应激指数(OSi)高于SRF-P2和PSRF(0.42 ± 0.06对0.23 ± 0.09和0.08 ± 0.00;P 结论:虽然精子DNA完整性在射精组分之间没有发现差异,但SRF-P1和SRF-P2表现出比PSRF更大的染色质凝聚。这可能与每个馏分的OSi有关。
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来源期刊
CiteScore
10.30
自引率
0.00%
发文量
822
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