Examination of pretreatment methods for DNA extraction from nails

IF 0.5 Q4 GENETICS & HEREDITY Forensic Science International: Genetics Supplement Series Pub Date : 2022-12-01 DOI:10.1016/j.fsigss.2022.09.039

Eriko Ochiai , Minoru Asogawa , Wataru Irie , Chizuko Sasaki , Naomi Nakamaru , Momoko Sakamoto , Junpei Nagato , Junichiro Saito , Masatoshi Sugisawa , Fumiko Satoh

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Abstract

In the conventional method of DNA extraction from nails, it takes approximately half a day to dissolve the nails. In this study, we examined whether using the HOrizontal Nail MAshing (HONMA) method, in which pressure is applied to the nail to crush it flat and increase its surface area, would improve DNA extraction efficiency. Fingernails (5 mg) provided by ten volunteers were used as samples. Nail pieces (1–3 pieces), shredded with nail clippers, were thinly stretched by applying 2 t of pressure to each piece using a hydraulic press. DNA was extracted by incubation at 56 °C for 10 min and 1 h during proteolysis. DNA yield from the nails pretreated using the HONMA method increased by 0.20–7.10 times compared with that from unprocessed nails. In particular, 10-min incubation using the HONMA method resulted in an average 2.05-fold increase in DNA yield compared with that under overnight incubation. However, the impact of using the HONMA method varied widely among individuals, and the amount of extracted DNA decreased in some cases, suggesting that the yield may differ depending on the nail quality.

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指甲DNA提取预处理方法的检验

在从指甲中提取DNA的传统方法中，溶解指甲大约需要半天时间。在这项研究中，我们检验了使用HOrizontal Nail MAshing（HONMA）方法是否会提高DNA提取效率，在该方法中，对指甲施加压力，使其变平并增加其表面积。使用10名志愿者提供的指甲（5mg）作为样本。用指甲刀切碎的指甲片（1-3片），通过使用液压机对每片施加2吨压力，将其薄薄地拉伸。在蛋白水解过程中，通过在56°C下孵育10分钟和1小时来提取DNA。使用HOMMA方法预处理的指甲的DNA产量比未处理的指甲增加了0.20–7.10倍。特别是，与过夜孵育相比，使用HOMMA方法孵育10分钟导致DNA产量平均增加2.05倍。然而，使用HOMMA方法的影响因个体而异，在某些情况下提取的DNA量减少，这表明产量可能因指甲质量而异。

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来源期刊

Forensic Science International: Genetics Supplement Series Medicine-Pathology and Forensic Medicine

CiteScore

0.40

自引率

0.00%

发文量

122

审稿时长

25 days

期刊介绍： The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.