DNA profiles from biological trace material contained in underwear and toothbrushes

IF 0.5 Q4 GENETICS & HEREDITY Forensic Science International: Genetics Supplement Series Pub Date : 2022-12-01 DOI:10.1016/j.fsigss.2022.10.045

Abdiel Gibran Torres-Pérez , Mauro López-Armenta , Alexa Villavicencio-Queijeiro

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Abstract

There are special cases where it is extremely difficult to find a close relative for obtaining reference samples, such as in adoption cases or foreigners without relatives in the country. In these cases, it is possible to use personal objects from the missing person. We developed a protocol for obtaining DNA profiles from toothbrushes and underwear and evaluated two DNA extraction methods (Silica and magnetic particles), comparing the DNA quantity and the quality of the obtained profiles. Results showed that for underwear, silica in suspension recovered higher DNA quantity but magnetic particles (PrepFiler®) generated more complete profiles. For toothbrushes PrepFiler® recovered a higher DNA quantity and generated more complete DNA profiles. Statistical analyses showed there were no significant differences between both methods. Higher DNA quantities were recovered from underwear samples, however toothbrushes generated higher quality in DNA profiles, without obtaining mixed profiles, concluding that toothbrushes are a better item than underwear. Considering that silica in suspension method is three-times cheaper than PrepFiler®, we propose it is possible to successfully obtain DNA profiles from personal objects using a low-cost DNA extract method.

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从内衣和牙刷的生物痕迹物质中提取DNA

在某些特殊情况下，很难找到近亲来获取参考样本，例如收养案件或在该国没有亲属的外国人。在这些情况下，可以使用失踪人员的个人物品。我们开发了一种从牙刷和内衣中获得DNA图谱的方案，并评估了两种DNA提取方法（二氧化硅和磁性颗粒），比较了获得图谱的DNA数量和质量。结果表明，对于内衣，悬浮液中的二氧化硅回收了更高的DNA量，但磁性颗粒（PrepFiler®）产生了更完整的图谱。对于牙刷，PrepFiler®回收了更高的DNA量，并生成了更完整的DNA图谱。统计分析表明，两种方法之间没有显著差异。从内衣样本中回收了更高的DNA量，但牙刷的DNA图谱质量更高，没有获得混合图谱，因此牙刷比内衣更好。考虑到悬浮二氧化硅法比PrepFiler®便宜三倍，我们建议使用低成本的DNA提取方法可以成功地从个人物品中获得DNA图谱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Forensic Science International: Genetics Supplement Series Medicine-Pathology and Forensic Medicine

CiteScore

0.40

自引率

0.00%

发文量

122

审稿时长

25 days

期刊介绍： The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.