1H, 13C, and 15N resonance assignments of SarA monomer from Staphylococcus aureus in complex with DNA

IF 0.8 4区 生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2023-07-05 DOI:10.1007/s12104-023-10140-8
Dihong Fu, Bo Duan, Xianzhi Dong, Bin Xia
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Abstract

SarA is a global transcription regulator in S. aureus which regulates the expression of over 120 genes related to quorum sensing, biofilm synthesis, drug resistance and many other important physiological processes during host infection. SarA can bind to the promoter region of agr and other target genes to activate or repress the transcription. The crystal structure of SarA uncovered a MarR protein-like conformation with two symmetrical winged helix domains, while its DNA binding mechanism is still unknown. We have constructed a monomeric DNA binding domain of SarA (SarAΔN19) for the study of the interaction between SarA and DNA with NMR spectroscopy. Here, we report the 1H, 13C and 15N NMR assignment of SarAΔN19/DNA complex which is the first step towards further structure and function analysis.

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金黄色葡萄球菌SarA单体与DNA复合物的1H、13C和15N共振归属
SarA是金黄色葡萄球菌的一种全局转录调节因子,在宿主感染期间调节120多个与群体感应、生物膜合成、耐药性和许多其他重要生理过程有关的基因的表达。SarA可以与agr和其他靶基因的启动子区结合,激活或抑制转录。SarA的晶体结构揭示了具有两个对称翼螺旋结构域的MarR蛋白样构象,而其DNA结合机制尚不清楚。我们构建了SarA的单体DNA结合结构域(SarAΔN19),用于NMR光谱研究SarA与DNA之间的相互作用。在此,我们报道了SarAΔN19/DNA复合物的1H、13C和15N NMR归属,这是进一步结构和功能分析的第一步。
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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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