Evaluation of SDS-coated iron nanostructure on the gene expression of bio surfactant-producing genes by Pseudomonas aeruginosa

IF 3.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Engineering in Life Sciences Pub Date : 2022-08-24 DOI:10.1002/elsc.202200002
Yaser Ahsani Arani, Zahra Noormohammadi, Behnam Rasekh, Fatemeh Yazdian, Hojjat kazemi
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引用次数: 5

Abstract

Bio surfactants are natural surfactants that induce emulsification, displacement, increased solubility, and mobility of hydrophobic organic compounds. In this study, the gene expression of biosurfactant production genes by Pseudomonas aeruginosa in the presence of sodium dodecyl sulfate coated iron nanostructure (Fe/SDS) were evaluated. Emulsification Index and Surface Tension reduction test to check stability and emulsification the rhamnolipid were done. Purification was evaluated using thin layer chromatography (TLC) and expression of rhlA, mvfR, lasR, rhlR genes was determined using q-PCR technique. Binding of nanoparticles to bio surfactants was confirmed by TEM. The best emulsification index, was by the sample that exposed to 1 mg/L Fe/SDS nanoparticles for 2 days. Rhamnolipid produced in the presence of nanoparticles had an acceptable ability to reduce surface tension. The Rf (retention factor) value obtained was 0.63 by chromatography. q-PCR results showed that the expression of rhlA, mvfR, lasR, rhlR genes was significantly increased in Fe/SDS treated cells, which indicates the significant positive effect (P < 0.05) of nanoparticles on biosurfactant production of treated cells. While, SDS and Fe alone were not affected significantly (P > 0.05) on the expression of these genes. Our findings indicated the importance of nanoparticles in increasing the expression of genes involved in the bio surfactant production pathway of Pseudomonas aeruginosa.

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SDS包被铁纳米结构对铜绿假单胞菌产生生物表面活性剂基因表达的影响
生物表面活性剂是一种天然表面活性剂,可诱导疏水性有机化合物的乳化、置换、增加溶解度和流动性。在本研究中,评估了铜绿假单胞菌在十二烷基硫酸钠包被的铁纳米结构(Fe/SDS)存在下产生生物表面活性剂的基因表达。通过乳化指数和表面张力降低试验考察了鼠李糖脂的稳定性和乳化性。用薄层色谱(TLC)评价纯化,并用q-PCR技术测定rhlA、mvfR、lasR、rhlR基因的表达。TEM证实了纳米颗粒与生物表面活性剂的结合。最佳乳化指数是通过将样品暴露于1mg/LFe/SDS纳米颗粒2天。在纳米颗粒存在下产生的鼠李糖脂具有可接受的降低表面张力的能力。通过色谱法获得的Rf(保留因子)值为0.63。q-PCR结果显示,在Fe/SDS处理的细胞中,rhlA、mvfR、lasR、rhlR基因的表达显著增加,这表明纳米颗粒对处理的细胞产生生物表面活性剂具有显著的正向作用(P<;0.05)。而SDS和Fe单独对这些基因的表达没有显著影响(P>0.05)。我们的发现表明了纳米颗粒在增加铜绿假单胞菌生物表面活性剂生产途径中基因表达方面的重要性。
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来源期刊
Engineering in Life Sciences
Engineering in Life Sciences 工程技术-生物工程与应用微生物
CiteScore
6.40
自引率
3.70%
发文量
81
审稿时长
3 months
期刊介绍: Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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