Immunological characterization of cell lines established from malignant and normal human urothelium

M. Vilien , H. Wolf , F. Rasmussen
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引用次数: 10

Abstract

Cell lines from normal or malignant human urothelium, described elsewhere by morphological, cell kinetic and genotypical criteria, have been characterized further by their response to antibody or lymphocyte mediated cytotoxicity in vitro. Four groups of cell lines were included: (1) two fast proliferating cell lines from normal urothelium, (2) three fast proliferating cell lines from transitional cell carcinoma (TCC) together with two transformed sublines of normal derived cell lines, (3) six slowly proliferating TCC lines, and (4) a line from squamous cell carcinoma. HLA antigen expression was demonstrated in the cell lines of groups 1 and 3, but not in lines from group 2 or 4. The sensitivity to spontaneous lymphocyte mediated cytotoxicity (SLMC) of cells in group 1 and 2 exceeded that of cells from group 3 by a factor of 8. The TCC line, HU 456, was more susceptible to SLMC than T 24. Specifically increased cytotoxicity of lymphocytes from patients suffering from interstitial cystitis (IC) against HU 609 from normal urothelium indicated that this line expresses tissue specific antigens, and at the same time that an immune reaction may be part of the pathogenesis of IC. By a sensitive test for antibody-dependent, cell-mediated cytotoxicity (ADCC) antibody activity against HU 456 was found in only one of nine TCC patients and none of five clinical controls. A pronounced non-disease-related blocking serum activity, frequently found, may account for some of the negative findings. The SLMC against HU 456 could be inhibited but not abolished by Fab fragments of rabbit anti-human immunoglobulin, indicating an ADCC mechanism as part of the SLMC.

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恶性和正常人尿路上皮细胞系的免疫学特性
来自正常或恶性人类尿路上皮的细胞系,在其他地方通过形态学、细胞动力学和基因型标准描述,已经通过其对抗体或淋巴细胞介导的体外细胞毒性的反应来进一步表征。包括四组细胞系:(1)两个来自正常尿路上皮的快速增殖细胞系,(2)三个来自移行细胞癌(TCC)的快速增殖的细胞系以及两个正常来源细胞系的转化亚系,(3)六个缓慢增殖的TCC系,和(4)一个来自鳞状细胞癌的细胞系。HLA抗原表达在第1组和第3组的细胞系中得到证实,但在第2组或第4组的细胞株中没有得到证实。第1组和第2组的细胞对自发淋巴细胞介导的细胞毒性(SLMC)的敏感性比第3组的细胞高出8倍。TCC系HU 456比T 24更易感染SLMC。来自间质性膀胱炎(IC)患者的淋巴细胞对来自正常尿路上皮的HU 609的特异性细胞毒性增加表明该系表达组织特异性抗原,同时免疫反应可能是IC发病机制的一部分。通过对抗体依赖性、细胞介导的针对HU 456的细胞毒性(ADCC)抗体活性的敏感测试,仅在9名TCC患者中的1名和5名临床对照中的1例中发现。经常发现的明显的非疾病相关的阻断血清活性可能是一些阴性结果的原因。兔抗人免疫球蛋白的Fab片段可以抑制但不能消除针对HU 456的SLMC,这表明ADCC机制是SLMC的一部分。
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