Ultrasound-mediated delivery of Pik3cb shRNA using magnetic nanoparticles for the treatment of in-stent restenosis in a rat balloon-injured model.

IF 1.9 4区 医学 Q2 BIOLOGY Journal of Radiation Research Pub Date : 2024-01-19 DOI:10.1093/jrr/rrad083
Yuhao Wang, Miao Li, Zongxiang Sheng, Hong Ran, Jing Dong, Lingling Fang, Pingyang Zhang
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Abstract

The aim of the present work was to examine the effect of polyethylene glycol (PEG)-coated superparamagnetic iron oxide (SPIO) nanoparticles carrying Pik3cb short hairpin RNA (shRNA) in the prevention of restenosis with the aid of ultrasound and a magnetic field. SPIO is a type of contrast agent used in medical imaging to enhance the visibility of specific tissues or organs. It consists of tiny iron oxide nanoparticles that can be targeted to specific areas of interest in the body. PEG-coated SPIO nanoparticles carrying Pik3cb shRNA (SPIO-shPik3cb) were prepared, and the particle size and zeta potential of PEG-coated SPIO nanoparticles with and without Pik3cb shRNA were examined. After a right common artery balloon-injured rat model was established, the rats were randomly divided into four groups, and the injured arteries were transfected with SPIO-shPik3cb, saline, SPIO-shcontrol and naked shRNA Pik3cb. During the treatment, each group was placed under a magnetic field and was transfected with the aid of ultrasound. Rats were sacrificed, and the tissue was harvested for analysis after 14 days. The results suggested that the mean particle size and zeta potential of SPIO-shPik3cbs were 151.45 ± 11 nm and 10 mV, respectively. SPIO-shPik3cb showed higher transfection efficiency and significantly inhibited the intimal thickening compared with naked Pik3cb shRNA in vascular smooth muscle cells (VSMCs) (*P < 0.05). Moreover, SPIO-shPik3cb could also significantly downregulate the expression of pAkt protein compared with naked Pik3cb shRNA. According to the results, SPIO-shPik3cb can remarkably inhibit the intimal thickening under a combination of magnetic field exposure and ultrasound.

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使用磁性纳米颗粒超声介导的Pik3cb-shRNA递送用于治疗大鼠球囊损伤模型中的支架内再狭窄。
本工作的目的是在超声和磁场的帮助下,检测聚乙二醇(PEG)包被的携带Pik3cb短发夹RNA(shRNA)的超顺磁性氧化铁(SPIO)纳米颗粒在预防再狭窄中的作用。SPIO是一种用于医学成像的造影剂,用于增强特定组织或器官的可见性。它由微小的氧化铁纳米颗粒组成,可以靶向身体特定的感兴趣区域。制备了携带Pik3cb-shRNA的PEG包被的SPIO纳米颗粒(SPIO-shPik3cb),并检测了带有和不带有Pik3cb-shRNA的PEG-包被的SPIO纳米颗粒的粒径和ζ电位。建立大鼠右总动脉球囊损伤模型后,将大鼠随机分为四组,用SPIO-shPik3cb、生理盐水、SPIO-shcontrol和裸shRNA-Pik3cb转染损伤动脉。在治疗过程中,将每组置于磁场下,并在超声的帮助下进行转染。处死大鼠,14天后采集组织进行分析。结果表明,SPIO-shPik3cbs的平均粒径和ζ电位为151.45 ± 11nm和10mV。SPIO-shPik3cb在血管平滑肌细胞(VSMCs)中显示出比裸Pik3cb-shRNA更高的转染效率,并显著抑制内膜增厚(*P
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来源期刊
CiteScore
3.60
自引率
5.00%
发文量
86
审稿时长
4-8 weeks
期刊介绍: The Journal of Radiation Research (JRR) is an official journal of The Japanese Radiation Research Society (JRRS), and the Japanese Society for Radiation Oncology (JASTRO). Since its launch in 1960 as the official journal of the JRRS, the journal has published scientific articles in radiation science in biology, chemistry, physics, epidemiology, and environmental sciences. JRR broadened its scope to include oncology in 2009, when JASTRO partnered with the JRRS to publish the journal. Articles considered fall into two broad categories: Oncology & Medicine - including all aspects of research with patients that impacts on the treatment of cancer using radiation. Papers which cover related radiation therapies, radiation dosimetry, and those describing the basis for treatment methods including techniques, are also welcomed. Clinical case reports are not acceptable. Radiation Research - basic science studies of radiation effects on livings in the area of physics, chemistry, biology, epidemiology and environmental sciences. Please be advised that JRR does not accept any papers of pure physics or chemistry. The journal is bimonthly, and is edited and published by the JRR Editorial Committee.
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