Lathyrane diterpenoids from Euphorbia lathyris induce cell-cycle arrest and apoptosis in human hypertrophic scar cells.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2024-12-01 Epub Date: 2023-11-10 DOI:10.1080/14786419.2023.2280736

Chun-Yan Zhang, Shun Xu, Lei Yu, Yi-Wen Nie, Si-Bo Li, Jian-Yong Zhu

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Abstract

One new lathyrane-type diterpenoid, euphlathin A (1), and 11 known analogues (2-12), were isolated from the fruits of Euphorbia lathyris. Their structures were elucidated by spectroscopic data. The absolute configurations of 1 were established by single-crystal X-ray crystallography. All diterpenoids (1-12) were evaluated for antiproliferative activity against the human hypertrophic scar (HTS) cells. Compound 1 exhibited significantly against HTS cells growth with an IC₅₀ value of 6.33 μM. Morphological features of apoptosis were evaluated in 1-treated HTS cells. Wound healing assays indicated that 1 significantly inhibited the migration of HTS at 24 h and 48 h. Compound 1 effectively induced apoptosis of HTS, which was associated with G₂/M or S phase cell cycle arrest. Flow cytometric analysis showed that the treatment by 1 significantly induced HTS cell apoptosis in a dose-dependent manner. Overall, euphlathin A (1) has the potential to be a therapeutic agent for the treatment of hyperplastic scar therapy.

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大戟中的Lathyrane二萜类化合物诱导人增生性瘢痕细胞的细胞周期停滞和凋亡。

从大戟果实中分离得到一个新的板条烷型二萜类化合物，即大板条素A（1）和11个已知的类似物（2-12）。通过光谱数据阐明了它们的结构。1的绝对构型是通过单晶X射线晶体学确定的。评估所有二萜类化合物（1-12）对人类增生性瘢痕（HTS）细胞的抗增殖活性。化合物1对HTS细胞生长表现出显著的抑制作用，IC50值为6.33 μM。在1处理的HTS细胞中评估细胞凋亡的形态学特征。伤口愈合试验表明，1在24小时时显著抑制HTS的迁移 h和48 h.化合物1有效地诱导HTS的凋亡，其与G2/M或S期细胞周期停滞有关。流式细胞仪分析显示，1以剂量依赖的方式显著诱导HTS细胞凋亡。总的来说，胡拉素A（1）有潜力成为治疗增生性瘢痕的治疗剂。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.