Effects of combined supplementation of vitamins C and E on the oxidative modification of low-density lipoprotein, soluble form of CD36, soluble vascular cell adhesion molecule-1, and nitrite/nitrate oxide levels in idiopathic nephrotic syndrome

Abstract

This study aimed to investigate whether a combined supplementation of vitamins C and E was able to modify the oxidized low-density lipoprotein (ox-LDL), soluble form of CD36 (sCD36), soluble vascular cell adhesion molecule-1 (sVCAM-1), and nitrite/nitrate oxide (NO_x) levels in pediatric nephrotic syndrome (NS) cases. The study included 36 children with NS. The patients were randomly allocated to either the treatment group or the placebo group (18 children each). The treatment group received a combined supplementation of vitamins C and E. The serum levels of ox-LDL, sCD36, and sVCAM-1 were assayed by enzyme-linked immunosorbent assay. The serum levels of NO_x were assayed by colorimetric assay. Results showed that the levels of ox-LDL, sVCAM-1, and NO_x were decreased after treatment with a combined supplementation of vitamins C and E, but there was no statistical difference (p > 0.05). After treatment, there was an increase in the level of sCD36 in both groups, although this was not significantly different (p > 0.05). The level of ox-LDL was significantly lower in the remission-idiopathic NS (remission-INS) group compared with the nonremission-INS group (p < 0.05). The levels of ox-LDL and sVCAM-1 were significantly lower in the remission-treated group than in the nonremission-treated group (p < 0.05). In conclusion, the combined supplementation of vitamins C and E cannot modify the ox-LDL, sCD36, sVCAM-1, and NO_x levels in children with INS. In remission cases, the combined supplementation of vitamins C and E reduces the ox-LDL and sVCAM-1 levels.