Evaluation of some blood parameters in the experimental autoimmune encephalomyelitis mouse model

Abstract

Multiple sclerosis (MS) is a chronic neuroinflammatory demyelinating disorder of the central nervous system with unclear exact etiology. The experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice is the most common animal model for MS sharing many clinical and pathophysiological features to expand our knowledge on the pathophysiology of the disease and to develop novel treatment strategies. The current study was designed to determine hematological parameters and plasma total protein (TP) and albumin (ALB) levels in EAE-in-duced C57BL/6 mice to help researchers working on the EAE animal model. EAE was induced with myelin oligodendrocyte glycoprotein (MOG35-55) peptide in the female C57BL/6 mice. The EAE clinically caused paralyzed tail, hind limb paresis, and uncoordinated movement in the mice. The EAE-induced mice hematologically had a statistically significant mild increase in white blood cell (WBC) count without altering neutrophil-lymphocyte ratio but no change in vital hematological parameters such as red blood cell count, packed cell volume, and hemoglobin level. Moreover, the EAE led to an increase in the plasma TP level and attenuation in plasma ALB level in the mice. In conclusion, our findings show that the EAE model in mice might not cause any significant change hematologically, except a slight increase in the WBC count, and might produce changes in the plasma protein level. As the findings of the current study, the EAE-induced blood parameter effects could consider understanding the pathophysiology of the disease and developing a novel therapeutic approach for the disease