Quantitative evaluation of light microscopes based on image processing techniques

L R van den Doel, A D Klein, S L Ellenberger, H Netten, F R Boddeke, L J van Vliet, I T Young
{"title":"Quantitative evaluation of light microscopes based on image processing techniques","authors":"L R van den Doel,&nbsp;A D Klein,&nbsp;S L Ellenberger,&nbsp;H Netten,&nbsp;F R Boddeke,&nbsp;L J van Vliet,&nbsp;I T Young","doi":"10.1002/1361-6374(199809)6:3<138::AID-BIO4>3.0.CO;2-8","DOIUrl":null,"url":null,"abstract":"<p>In this note we will present methods based on image processing techniques to evaluate the performance of light microscopes. These procedures are applied to three different ‘high-end’ light microscopes. Tests are carried out to measure the homogeneity of the illumination system. From these tests it follows that Köhler illuminated images can have an exceedingly high amount of shading. Another result found from the illumination calibration is that traditional lamp housings are not designed to make fine-tuning easy. Next, the (automated) stage is considered. Several tests are performed to measure the stage motion in the <i>xy</i>-plane and in the axial direction to address accuracy, precision, and hysteresis effects. Finally, the entire electro-optical system is characterized by measuring the optical transfer function (OTF) at wavelengths 400 nm, 500 nm, 600 nm, and 700 nm. The results of these experiments show that there is a consistent deviation from the theoretical OTF at wavelengths around 400 nm. The final conclusion is that modern light microscopes perform better than their five-to-ten-year-old predecessors.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199809)6:3<138::AID-BIO4>3.0.CO;2-8","citationCount":"20","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199809%296%3A3%3C138%3A%3AAID-BIO4%3E3.0.CO%3B2-8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 20

Abstract

In this note we will present methods based on image processing techniques to evaluate the performance of light microscopes. These procedures are applied to three different ‘high-end’ light microscopes. Tests are carried out to measure the homogeneity of the illumination system. From these tests it follows that Köhler illuminated images can have an exceedingly high amount of shading. Another result found from the illumination calibration is that traditional lamp housings are not designed to make fine-tuning easy. Next, the (automated) stage is considered. Several tests are performed to measure the stage motion in the xy-plane and in the axial direction to address accuracy, precision, and hysteresis effects. Finally, the entire electro-optical system is characterized by measuring the optical transfer function (OTF) at wavelengths 400 nm, 500 nm, 600 nm, and 700 nm. The results of these experiments show that there is a consistent deviation from the theoretical OTF at wavelengths around 400 nm. The final conclusion is that modern light microscopes perform better than their five-to-ten-year-old predecessors.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
基于图像处理技术的光学显微镜定量评价
在这篇文章中,我们将介绍基于图像处理技术的方法来评估光学显微镜的性能。这些程序适用于三种不同的“高端”光学显微镜。进行了测试以测量照明系统的均匀性。从这些测试可以得出Köhler照明图像可以有非常高的阴影量。从照明校准中发现的另一个结果是,传统的灯具外壳设计不容易进行微调。接下来,考虑(自动化)阶段。为了解决精度、精度和迟滞效应,在x平面和轴向上进行了几个测试来测量舞台运动。最后,通过测量400 nm、500 nm、600 nm和700 nm波长处的光学传递函数(OTF)对整个电光系统进行了表征。实验结果表明,在400 nm左右的波长处与理论OTF存在一致的偏差。最后的结论是,现代光学显微镜比5到10年前的前辈表现得更好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Magnetic Particle Imaging Magnetic Resonance Imaging Quantitative evaluation of light microscopes based on image processing techniques Confocal microscopy of single molecules of the green fluorescent protein Heavy metal contrast enhancement for the selective detection of gold particles in electron microscopical sections using electron spectroscopic imaging
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1