Transcutaneous biochemical substance monitoring based on biosensors-blood glucose and lactate

Abstract

New transcutaneous monitoring methods for biochemical substances such as blood glucose and lactate have been developed by using a suction effusion fluid (SEF) collecting technique with an ion-sensitive field-effect transistor for blood glucose, and with a micro-planar amperometric biosensor for lactate respectively. Firstly, blood glucose change during the 75-g oral glucose tolerance test (OGTT) was monitored by suction effusion fluid (SEF) sampling in 5 patients with type II diabetes. Secondly, the authors have also developed a micro planar biosensor for determining lactate concentration in very small sample. The biosensor utilizes a lactate oxidase immobilized membrane on a micro planar platinum electrode to yield a lactate sensor. Both glucose and lactate concentrations measured with SEF showed good correlation with those obtained directly from whole blood.