Copper to Ceruloplasmin Ratio During Normal Pregnancy In Osogbo, Nigeria.

D. Oparinde, C. Ezeogu, A. Okesina, A. Adeniji, A. Iyanda, A. Atiba
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引用次数: 3

Abstract

Disordered copper and ceruloplasmin levels have been associated in pathologic pregnancies, however, investigation of copper status especially the determination of free copper which is the physiologically active fraction has been met with some difficulties. In this regard, the determination of copper/ceruloplasmin ratio has been proposed as one of the better indicators of copper status in pregnancy. Since copper/ceruloplasmin ratio varies from region to region, it has been advocated that each region determines its own cut-off. We therefore determined copper/ceruloplasmin ratio during normal pregnancy in Osogbo, SouthWestern Nigeria. 150 consenting pregnant women attending the antenatal Clinic of Ladoke Akintola University of Technology Teaching Hospital in different stages of gestation were recruited. Another 35 non pregnant age matched mothers were recruited as controls. 5 mls of venous blood was obtained from subjects and controls for the determination of serum copper with the aid of atomic absorption spectrophotometer and serum ceruloplasmin determined with a method based on its p- Phenylene Diamine Oxidase Activity according to the colourimetric method of Sunderman and Nomoto Copper in the first, second and third trimesters of pregnancy increases steadily and significantly compared with non pregnant subjects (36.72±3.75,46.88±0.02 and 58.13±2.50vs14.43±4.50imol/l, p significantly elevated above non pregnant controls, 47.21±8.88imol/l vs. 14.43±4.50imol/l p third trimesters increased significantly from each trimester and were significantly elevated above that of c o n t r o l s ( 4 9 . 5 6 ± 5 . 1 4 , 7 1 . 7 7 ± 7 . 0 7 a n d 90.70±2.77mg/100ml vs. 35.62±2.55mg/100ml, p trimester is significantly higher than in second and third trimesters while copper/ceruloplasmin ratios during second and third trimesters were similar. However, copper/ceruloplasmin ratio during first, second and third trimesters are significantly higher than in controls, 0.074 imol/g, 0.065 imol/g and 0.064 imol/g vs. 0.041 imol/g. Average copper/ceruloplasmin ratio in the total pregnant population was significantly higher than in controls 0.067 imol/g vs. 0.041 imol/g. Copper/ceruloplasmin ratio is highest during the first trimester and gradually decreases as pregnancy advances, this decrease may imply a decrease in available free copper to the fetus. Therefore a molecule of ceruloplasmin containing less than 0.064 imol/g of copper may suggest excess product ion of ceruloplasmin in relation to available copper which has been associated with pathologic pregnancies especially pre-eclampsia and eclampsia.
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在尼日利亚奥索博正常怀孕期间铜与铜蓝蛋白的比例。
病理性妊娠中铜和铜蓝蛋白水平紊乱,但对铜状态的研究,特别是对生理活性部分游离铜的测定,遇到了一些困难。在这方面,铜/铜蓝蛋白比的测定被认为是妊娠期铜状态较好的指标之一。由于铜/铜蓝蛋白的比例因地区而异,因此一直主张每个地区确定自己的截止值。因此,我们在尼日利亚西南部的Osogbo测定了正常妊娠期间铜/铜蓝蛋白的比例。我们招募了150名在不同妊娠阶段在Ladoke Akintola科技大学教学医院产前门诊就诊的孕妇。另外35名未怀孕的年龄相匹配的母亲被招募作为对照。分别取被试和对照组静脉血5 ml,用原子吸收分光光度计测定血清铜,用对苯二胺氧化酶活性法测定血清铜蓝蛋白,方法参照第一篇中Sunderman和Nomoto copper的比色法。妊娠中期和晚期与未妊娠组比较,p值稳定且显著升高(36.72±3.75、46.88±0.02和58.13±2.50vs14.43±4.50imol/l, p值显著高于未妊娠组,p值分别为47.21±8.88imol/l和14.43±4.50imol/l), p值在妊娠中期和晚期均显著升高,且显著高于未妊娠组。5±5。1 4 7 1。7±7。妊娠中期和晚期铜/铜蓝蛋白比值均显著高于妊娠中期和晚期,而妊娠中期和晚期铜/铜蓝蛋白比值相似。然而,铜/铜蓝蛋白比值在妊娠早期、中期和晚期显著高于对照组,分别为0.074 imol/g、0.065 imol/g和0.064 imol/g,对照组为0.041 imol/g。妊娠人群铜/铜蓝蛋白平均比值显著高于对照组0.067 imol/g和0.041 imol/g。铜/铜蓝蛋白比率在妊娠早期最高,随着妊娠的进展逐渐降低,这种降低可能意味着胎儿可利用的游离铜的减少。因此,铜蓝蛋白分子中铜含量低于0.064 imol/g可能表明铜蓝蛋白的过量生成与病理性妊娠特别是子痫前期和子痫有关。
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