Determination of α,4-dihydroxy-3-methoxybenzeneacetic acid (vanilmandelic acid) by flow injection analysis coupled with luminol–hexacyanoferrate(III) chemiluminescence detection

N. W. Barnett, P. S. Francis, S. Lewis, K. Lim
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引用次数: 24

Abstract

A simple and robust method for the determination of the clinically significant molecule vanilmandelic acid (α,4-dihydroxy-3-methoxybenzeneacetic acid, 4-hydroxy-3-methoxymandelic acid) using flow injection analysis with chemiluminescence detection is described. This compound was found to enhance the chemiluminescence from the reaction of luminol with hexacyanoferrate(III) in a basic medium. The calibration function obtained over the range from 2.5 × 10–8 to 1.0 × 10–6 mol dm–3 approximated to linearity (r2 = 0.9995) with the equation of best fit of y = 1.72 × 108x + 36.9, where y is the integrated signal (V s) and x is the concentration (mol dm–3). Precision (as measured by relative standard deviation) was 2.6% for 5 replicate analyses of a standard (2.5 × 10–7 mol dm–3) and the limit of detection (3σ) was 2 × 10–8 mol dm–3. Preliminary investigations revealed that the structurally similar homovanillic acid inhibits the emission of chemiluminescence from the luminol–hexacyanoferrate(III) reaction and that the kinetics of light emission were different from those of the vanilmandelic acid enhanced reaction.
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流动注射联用鲁米诺-六氰铁酸酯(III)化学发光法测定α,4-二羟基-3-甲氧基苯乙酸(香草酸)
本文建立了一种简便、可靠的流动注射化学发光法测定临床重要分子香蓝桃酸(α,4-二羟基-3-甲氧基苯乙酸,4-羟基-3-甲氧基香蓝桃酸)的方法。该化合物在碱性介质中与六氰高铁酸盐(III)反应时增强了化学发光。在2.5 × 10-8 ~ 1.0 × 10-6 mol dm-3范围内得到的校准函数近似线性(r2 = 0.9995),最佳拟合方程为y = 1.72 × 108x + 36.9,其中y为积分信号(V s), x为浓度(mol dm-3)。对一个标准品(2.5 × 10-7 mol dm-3)进行5次重复分析,精密度(以相对标准偏差测量)为2.6%,检出限(3σ)为2 × 10-8 mol dm-3。初步研究表明,结构相似的同香草酸抑制鲁米诺-六氰铁酸盐(III)反应的化学发光,其发光动力学与香草酸增强反应的发光动力学不同。
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