{"title":"Isolation and characterization of water-soluble fractions of black sesame pigment and its antioxidant activities in vitro","authors":"Jihong WU , Qian HUANG , Shuang ZHU , Sharui SHAN , Jinmei HU , Mehmood Abbasi ARSHAD , Lin ZHOU","doi":"10.1016/S2707-3688(23)00056-0","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Black sesame pigment (BSP) was a natural pigment with a variety of bioactivities, including antioxidant, antimutagenic, and neuroprotective properties. However, the high molecular weight and heterogeneous structure of BSP necessitate further investigation. The study's goal is to develop a theoretical foundation for water-soluble BSP as a potential functional food supplement.</p></div><div><h3>Methods</h3><p>First, crude black sesame pigment (rBSP) was extracted from black sesame by sodium hydroxide-hydrochloric acid method, furtherly two water-soluble components (BSP-1, BSP-2) were prepared from rBSP by the separation methods of macroporous adsorption resin. In the dynamic test, 40% ethanol (<em>v/v</em>) was applied to five different macroporous resins (AB-8, D101, XAD-1600, D201, and HPD-600) for static adsorption/desorption of rBSP separation. The structure of the components was investigated using ultraviolet (UV) spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), and <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance (NMR). Oxygen radical absorption capacity (ORAC) and cellular antioxidation activities (CAA) were used to assess the antioxidant activity of the components.</p></div><div><h3>Results</h3><p>A macroporous polymeric adsorbent method was developed to obtain water-soluble fractions of rBSP. For the static adsorption/desorption characteristics of rBSP, five types of macroporous resins (AB-8, D101, XAD-1600, D201, and HPD-600) were evaluated, and two fractions (BSP-1 and BSP-2) were isolated from rBSP by 40% ethanol (<em>v/v</em>) on D101 resin in the dynamic test. Furthermore, ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, <sup>1</sup>H and <sup>13</sup>C NMR revealed that BSP-1 and BSP-2 were macromolecule conjugate structures, with BSP-2 having higher aromaticity than BSP-1. ORAC and CAA results showed that rBSP, BSP-1, and BSP-2 had comparable antioxidant activities. The BSP-2 exhibited the highest CAA value of 1121.92 ± 54.45 µmol QE/100 g, DW.</p></div><div><h3>Conclusion</h3><p>This study suggests that water-soluble BSP fractions could be used as functional food supplements that benefit human health.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"3 2","pages":"Pages 163-176"},"PeriodicalIF":0.0000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823000560/pdfft?md5=a28ecdee583fdb59bd0efcc286d9974c&pid=1-s2.0-S2707368823000560-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Holistic Integrative Pharmacy","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2707368823000560","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective
Black sesame pigment (BSP) was a natural pigment with a variety of bioactivities, including antioxidant, antimutagenic, and neuroprotective properties. However, the high molecular weight and heterogeneous structure of BSP necessitate further investigation. The study's goal is to develop a theoretical foundation for water-soluble BSP as a potential functional food supplement.
Methods
First, crude black sesame pigment (rBSP) was extracted from black sesame by sodium hydroxide-hydrochloric acid method, furtherly two water-soluble components (BSP-1, BSP-2) were prepared from rBSP by the separation methods of macroporous adsorption resin. In the dynamic test, 40% ethanol (v/v) was applied to five different macroporous resins (AB-8, D101, XAD-1600, D201, and HPD-600) for static adsorption/desorption of rBSP separation. The structure of the components was investigated using ultraviolet (UV) spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), and 1H and 13C nuclear magnetic resonance (NMR). Oxygen radical absorption capacity (ORAC) and cellular antioxidation activities (CAA) were used to assess the antioxidant activity of the components.
Results
A macroporous polymeric adsorbent method was developed to obtain water-soluble fractions of rBSP. For the static adsorption/desorption characteristics of rBSP, five types of macroporous resins (AB-8, D101, XAD-1600, D201, and HPD-600) were evaluated, and two fractions (BSP-1 and BSP-2) were isolated from rBSP by 40% ethanol (v/v) on D101 resin in the dynamic test. Furthermore, ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, 1H and 13C NMR revealed that BSP-1 and BSP-2 were macromolecule conjugate structures, with BSP-2 having higher aromaticity than BSP-1. ORAC and CAA results showed that rBSP, BSP-1, and BSP-2 had comparable antioxidant activities. The BSP-2 exhibited the highest CAA value of 1121.92 ± 54.45 µmol QE/100 g, DW.
Conclusion
This study suggests that water-soluble BSP fractions could be used as functional food supplements that benefit human health.
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