Journal of Holistic Integrative Pharmacy最新文献

英文中文

Development of ginger oleoresin-enriched marshmallow candy as a nutraceutical for managing pediatric chemotherapy-induced nausea and vomiting

Journal of Holistic Integrative Pharmacy

Pub Date : 2025-03-01 DOI: 10.1016/j.jhip.2025.02.002

Marzooka Kazi-Chishti , Umme Jasvi Kulsum , Mohamed Hassan Dehghan , Mohd Nazimuddin Chishti , Kazi Bilal

Objective

Chemotherapy-induced nausea and vomiting (CINV) significantly impact pediatric cancer patients, affecting treatment adherence and quality of life. This study aimed to develop gingerol-enriched marshmallow candy as a nutraceutical to alleviate CINV, offering a palatable and effective antiemetic formulation for children.

Methods

A central composite experimental design was employed to optimize the formulation. The various parameters, including textural attributes (hardness, springiness, and cohesiveness), weight variation, disintegration time, in vitro release, moisture content, water activity coefficient, and stability, of the marshmallows were evaluated to ensure the efficacy and quality of the product.

Results

The study identified an optimal formulation comprising ginger powder extract (4% w/w), gelatin (6% w/w), gum acacia (2.5% w/w), and agar (2.5% w/w). This composition demonstrated excellent textural characteristics, rapid disintegration, and efficient gingerol release in simulated conditions. The marshmallow candy also exhibited high acceptability in terms of stability and potential usability as a pediatric nutraceutical.

Conclusion

The ginger oleoresin-enriched marshmallow candy presents a novel and appealing delivery system for managing CINV in pediatric patients. Its favorable sensory and functional properties could improve compliance and enhance the overall treatment experience for children undergoing chemotherapy.

{"title":"Development of ginger oleoresin-enriched marshmallow candy as a nutraceutical for managing pediatric chemotherapy-induced nausea and vomiting","authors":"Marzooka Kazi-Chishti , Umme Jasvi Kulsum , Mohamed Hassan Dehghan , Mohd Nazimuddin Chishti , Kazi Bilal","doi":"10.1016/j.jhip.2025.02.002","DOIUrl":"10.1016/j.jhip.2025.02.002","url":null,"abstract":"<div><h3>Objective</h3><div>Chemotherapy-induced nausea and vomiting (CINV) significantly impact pediatric cancer patients, affecting treatment adherence and quality of life. This study aimed to develop gingerol-enriched marshmallow candy as a nutraceutical to alleviate CINV, offering a palatable and effective antiemetic formulation for children.</div></div><div><h3>Methods</h3><div>A central composite experimental design was employed to optimize the formulation. The various parameters, including textural attributes (hardness, springiness, and cohesiveness), weight variation, disintegration time, <em>in vitro</em> release, moisture content, water activity coefficient, and stability, of the marshmallows were evaluated to ensure the efficacy and quality of the product.</div></div><div><h3>Results</h3><div>The study identified an optimal formulation comprising ginger powder extract (4% w/w), gelatin (6% w/w), gum acacia (2.5% w/w), and agar (2.5% w/w). This composition demonstrated excellent textural characteristics, rapid disintegration, and efficient gingerol release in simulated conditions. The marshmallow candy also exhibited high acceptability in terms of stability and potential usability as a pediatric nutraceutical.</div></div><div><h3>Conclusion</h3><div>The ginger oleoresin-enriched marshmallow candy presents a novel and appealing delivery system for managing CINV in pediatric patients. Its favorable sensory and functional properties could improve compliance and enhance the overall treatment experience for children undergoing chemotherapy.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 1","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer flavonoids producing endophytic fungi: A review

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.11.002

Kirti G. Sahu , Deepak S. Khobragade , Shriniwas P. Patil

Plants living in different environments inhabit endophytic microbes. The relationship between plants and endophytic microbes may be symbiotic or parasitic. Transmission of endophytes varies when they are obtained through roots or cracks on bark or airborne spores from soil or originated from seeds or pollens. Endophytes could be bacteria or fungi. So far, actual flavonoids have been detected from endophytic fungi only. Several flavonoids like rutin, quercetin, kaempferol, naringenin, vitexin, apigenin, luteolin, chrysin, and silymarin are isolated from endophytic fungi. These flavonoids are tested for their anticancer activity on different cell lines and their mechanisms were determined. This review focuses on endophytic fungi from which anticancer flavonoids have been reported.

引用次数: 0

Visual analysis of drug research and development based on artificial intelligence

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.12.002

Wei Wei , Chao Song , Changxing Qi , Xin Zhang , Xiaoyi Zhang , Run Pu , Yi Ao

The iteration of artificial intelligence (AI) technology provides new opportunities for drug research and experimental development. In recent years, AI-based drug research has continuously made new progress and has garnered widespread attention. This study retrieved data from a total of 23,096 papers in AI-based drug research and development from the Web of Science up to May 14, 2024, and conducted bibliometric analysis using VOSviewer software. The results indicated that the AI-based drug research and development is a globally recognized hotspot, and the United States holds a certain authority in this field, while China ranks second in total publication output. The integration of AI technology with drug development primarily involves four stages: drug discovery, preclinical research, clinical trials, and drug manufacturing. So, AI technology has been applied throughout the entire process of drug development. AI-based virtual drug screening and structure-activity relationship analysis started early, while graph neural networks, pre-trained models (Transformer), interpretable AI technology, ChatGPT, and large language models were significantly highlighted in the last 3 years. Moreover, since 2020, AI-based drug repurposing, molecular dynamics simulation, 3D printing, and drug delivery system design have emerged as research hotspots and have been mainly applied to, particularly, on COVID-19, disease prognosis, liver cancer, lung cancer, and immunotherapy.

{"title":"Visual analysis of drug research and development based on artificial intelligence","authors":"Wei Wei , Chao Song , Changxing Qi , Xin Zhang , Xiaoyi Zhang , Run Pu , Yi Ao","doi":"10.1016/j.jhip.2024.12.002","DOIUrl":"10.1016/j.jhip.2024.12.002","url":null,"abstract":"<div><div>The iteration of artificial intelligence (AI) technology provides new opportunities for drug research and experimental development. In recent years, AI-based drug research has continuously made new progress and has garnered widespread attention. This study retrieved data from a total of 23,096 papers in AI-based drug research and development from the Web of Science up to May 14, 2024, and conducted bibliometric analysis using VOSviewer software. The results indicated that the AI-based drug research and development is a globally recognized hotspot, and the United States holds a certain authority in this field, while China ranks second in total publication output. The integration of AI technology with drug development primarily involves four stages: drug discovery, preclinical research, clinical trials, and drug manufacturing. So, AI technology has been applied throughout the entire process of drug development. AI-based virtual drug screening and structure-activity relationship analysis started early, while graph neural networks, pre-trained models (Transformer), interpretable AI technology, ChatGPT, and large language models were significantly highlighted in the last 3 years. Moreover, since 2020, AI-based drug repurposing, molecular dynamics simulation, 3D printing, and drug delivery system design have emerged as research hotspots and have been mainly applied to, particularly, on COVID-19, disease prognosis, liver cancer, lung cancer, and immunotherapy.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 323-332"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the application of Aloe vera in cosmetology and clinical treatment of skin diseases

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.11.006

Jiajun Zhu , Yuanru Zheng , Yanhui Ge

The medicinal value of Aloe vera (AV) has been continuously explored and utilized in recent years, becoming a research hotspot. Some basic studies have found that AV had cosmetic effects such as whitening, sun protection, antioxidant, anti-aging, and moisturizing. Some clinical trials have found that AV had medical effects such as antibacterial, anti-damage, and promoting wound healing. In this work, we summarized the indications and therapeutic efficacy of AV in cosmetology and clinical treatment of dermatosis, in order to provide certain inspiration for the development of new AV-based skincare products and new medicines for skin diseases.

引用次数: 0

Human Epididymis Protein 4 (HE4) as a promising biomarker and therapy target in fibrotic diseases: A review

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.12.001

Huiqun Tian, Li Chen

The treatment of fibrosis faces a significant challenge due to the lack of effective therapies that can reverse established fibrosis. Early detection is vital for intervention, yet distinguishing fibrosis from normal tissue repair is complex. Human Epididymis Protein 4 (HE4), a traditional tumor marker, has been found to be increased in some non-neoplastic conditions, such as fibrosis related diseases. According to properties analysis, HE4 has been characterized as a highly stable cross-class protease inhibitor, which interacts with key fibrotic proteins (such as MMP2 and PRSS family members) and potentially involves in the progression of fibrosis by inhibiting the enzymatic activity of these proteins. Meanwhile, studies indicated that HE4 may be involved in fibrosis through PI3K/AKT, NF-κB, MAPK, and other signaling pathways. Here we summarized the latest research progress of HE4 in pulmonary fibrosis, renal fibrosis, myocardial fibrosis, liver fibrosis, and autoimmune diseases induced fibrosis. As reported in this review, HE4 was closely related to disease severity and prognosis, and also was a promising prognostic evaluation marker and therapeutic intervention target for fibrotic diseases.

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引用次数: 0

Research on the role and mechanism of Aloe vera (L.) Burm.f. in the treatment of burn: Based on network pharmacology analysis and experimental verification

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.11.001

Yixiang Wu , Xiaoshan Zheng , Youchaou Mobet , Huiqun Tian , Fangsen Li , Huan Li , Lifeng Xie , Yanyue Deng , Xiaodi Zhu , Chuxi Tang , Hongwei Shao , Song Liu

Burn is a suddenly occurring injury. The healing process of burn is complex. Aloe vera (L.) Burm.f. (Av) is widely used as Chinese folk medicine for the treatment of burn. While effective pharmacological components, role and mechanism of Av in the treatment of burn remain to be fully elucidated. Arachidonic Acid (AA) and Quercetin (QUE) are identified as active components of Av for the treatment of burn by network pharmacology analysis and experimental verification. Animal experiments’ results showed that AA and QUE could shorten the burn wound healing time. The expression of inflammatory factor TNF-α, MyD88 and P-NF-κB p65 were down-regulated and the ER (ESR1), SRC were up-regulated in AA treated NIH 3T3 cells. The expression of MyD88 was downregulated in QUE treated NIH 3T3 cells. The Av promotes burn healing may be via the anti-inflammatory effect and regulation on NF-κB signal pathway or estrogen signal pathway by its active components AA and QUE. This research may construct a bridge between Av and burn wounds, and skin therapy agent exploration.

{"title":"Research on the role and mechanism of Aloe vera (L.) Burm.f. in the treatment of burn: Based on network pharmacology analysis and experimental verification","authors":"Yixiang Wu , Xiaoshan Zheng , Youchaou Mobet , Huiqun Tian , Fangsen Li , Huan Li , Lifeng Xie , Yanyue Deng , Xiaodi Zhu , Chuxi Tang , Hongwei Shao , Song Liu","doi":"10.1016/j.jhip.2024.11.001","DOIUrl":"10.1016/j.jhip.2024.11.001","url":null,"abstract":"<div><div>Burn is a suddenly occurring injury. The healing process of burn is complex. <em>Aloe vera</em> (L.) Burm.f. (Av) is widely used as Chinese folk medicine for the treatment of burn. While effective pharmacological components, role and mechanism of Av in the treatment of burn remain to be fully elucidated. Arachidonic Acid (AA) and Quercetin (QUE) are identified as active components of Av for the treatment of burn by network pharmacology analysis and experimental verification. Animal experiments’ results showed that AA and QUE could shorten the burn wound healing time. The expression of inflammatory factor TNF-α, MyD88 and P-NF-κB p65 were down-regulated and the ER (ESR1), SRC were up-regulated in AA treated NIH 3T3 cells. The expression of MyD88 was downregulated in QUE treated NIH 3T3 cells. The Av promotes burn healing may be via the anti-inflammatory effect and regulation on NF-κB signal pathway or estrogen signal pathway by its active components AA and QUE. This research may construct a bridge between Av and burn wounds, and skin therapy agent exploration.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 262-276"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pedalitin could regulate lipid metabolism and attenuate inﬂammatory factors in a non-alcoholic fatty liver disease cell model

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.11.003

Wanying He , Yaying Yu , Hui He , Qian Huang , Zhiting Liu , Fan Yang , Lin Zhou

Nonalcoholic fatty liver disease (NAFLD) is an escalating global health issue, leading to liver fat accumulation, inflammation, fibrosis, and potential liver failure. In our preliminary network pharmacological study, it was hypothesized that Pedalitin (PED), a flavonoid found in black sesame plants, might exhibit protective effects against NAFLD. However, the effects and mechanisms of PED underlying its action on NAFLD are not yet fully understood. This study aimed to explore the potential effects and mechanisms of PED on NAFLD using a combination of network pharmacology, molecular docking, and the LO2 cell model. Potential targets for PED and NAFLD were predicted through public databases. Protein-protein interaction (PPI) networks were constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. Molecular docking was used to predict the target genes that could bind to PED. In vitro experiments using the LO2 cell model indicated that PED significantly reduced TG level (p < 0.05) and the formation of lipid droplets. The expression levels of key factors in fatty acid metabolism (CPT2, HADH), inflammatory factors (IL-17, TNF-α), and the FOXO signaling pathway (EGFR, IRS1, AKT1, FOXO1) were significantly downregulated in LO2 cells treated with PED (p < 0.05). In conclusion, PED may modulate local lipid metabolism and mitigate inflammatory responses through the FOXO signaling pathway.

{"title":"Pedalitin could regulate lipid metabolism and attenuate inﬂammatory factors in a non-alcoholic fatty liver disease cell model","authors":"Wanying He , Yaying Yu , Hui He , Qian Huang , Zhiting Liu , Fan Yang , Lin Zhou","doi":"10.1016/j.jhip.2024.11.003","DOIUrl":"10.1016/j.jhip.2024.11.003","url":null,"abstract":"<div><div>Nonalcoholic fatty liver disease (NAFLD) is an escalating global health issue, leading to liver fat accumulation, inflammation, fibrosis, and potential liver failure. In our preliminary network pharmacological study, it was hypothesized that Pedalitin (PED), a flavonoid found in black sesame plants, might exhibit protective effects against NAFLD. However, the effects and mechanisms of PED underlying its action on NAFLD are not yet fully understood. This study aimed to explore the potential effects and mechanisms of PED on NAFLD using a combination of network pharmacology, molecular docking, and the LO2 cell model. Potential targets for PED and NAFLD were predicted through public databases. Protein-protein interaction (PPI) networks were constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. Molecular docking was used to predict the target genes that could bind to PED. <em>In vitro</em> experiments using the LO2 cell model indicated that PED significantly reduced TG level (<em>p</em> < 0.05) and the formation of lipid droplets. The expression levels of key factors in fatty acid metabolism (CPT2, HADH), inflammatory factors (IL-17, TNF-α), and the FOXO signaling pathway (EGFR, IRS1, AKT1, FOXO1) were significantly downregulated in LO2 cells treated with PED (<em>p</em> < 0.05). In conclusion, PED may modulate local lipid metabolism and mitigate inflammatory responses through the FOXO signaling pathway.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 314-322"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enlightenment of EU Herbal Medicinal Products regulation on the registration of Traditional Chinese Medicines in the Guangdong-Hong Kong-Macao Greater Bay Area

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.11.005

Ran Xiong , Hui Zhang , Yueyun Li , Guihao Zeng , Yonghui Liu , Yeyou Xu

Objective

This study analyzes the European Union's (EU's) regulatory practices of Herbal Medicinal Products (HMPs), aiming to provide implications for Traditional Chinese Medicines (TCMs) registration process within the Guangdong-Hong Kong-Macao Greater Bay Area (GBA).

Methods

This paper conducts a comparative analysis of the regulatory agencies, legal frameworks, registration classifications, market authorization procedures, and the number of applications and approvals for both HMPs in the EU and TCMs in the GBA, thereby identifying the distinctive regulatory features across these regions.

Results

Our study finds that the EU has established a scientific, efficient, and harmonized regulation system for HMPs, whereas the registration of TCMs in the GBA is characterized by complexity and jurisdictional disparities in requirements and procedures. Drawing on the EU's advanced regulatory practices, the study offers recommendations to enhance the mutual recognition and accessibility of TCMs within the GBA.

Conclusion

This study offers a comprehensive understanding of the EU's HMPs regulation and the GBA's TCMs registration, contributing to the synergistic development of TCMs in the GBA.

{"title":"Enlightenment of EU Herbal Medicinal Products regulation on the registration of Traditional Chinese Medicines in the Guangdong-Hong Kong-Macao Greater Bay Area","authors":"Ran Xiong , Hui Zhang , Yueyun Li , Guihao Zeng , Yonghui Liu , Yeyou Xu","doi":"10.1016/j.jhip.2024.11.005","DOIUrl":"10.1016/j.jhip.2024.11.005","url":null,"abstract":"<div><h3>Objective</h3><div>This study analyzes the European Union's (EU's) regulatory practices of Herbal Medicinal Products (HMPs), aiming to provide implications for Traditional Chinese Medicines (TCMs) registration process within the Guangdong-Hong Kong-Macao Greater Bay Area (GBA).</div></div><div><h3>Methods</h3><div>This paper conducts a comparative analysis of the regulatory agencies, legal frameworks, registration classifications, market authorization procedures, and the number of applications and approvals for both HMPs in the EU and TCMs in the GBA, thereby identifying the distinctive regulatory features across these regions.</div></div><div><h3>Results</h3><div>Our study finds that the EU has established a scientific, efficient, and harmonized regulation system for HMPs, whereas the registration of TCMs in the GBA is characterized by complexity and jurisdictional disparities in requirements and procedures. Drawing on the EU's advanced regulatory practices, the study offers recommendations to enhance the mutual recognition and accessibility of TCMs within the GBA.</div></div><div><h3>Conclusion</h3><div>This study offers a comprehensive understanding of the EU's HMPs regulation and the GBA's TCMs registration, contributing to the synergistic development of TCMs in the GBA.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 344-353"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the mechanism of Lingbao Huxin Dan in the treatment of bradycardia based on atrioventricular node electrical signal conduction

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.12.003

Jing Zhang , Guobin Zheng , Shangjing Liu , Yaodong Miao , Shu Yang , Sheng Li , Zhihui Yang , Danping Zhuo , Rui Guo , Yang Guo , Rongjiang Shao , Yunqing Hua , Chuanrui Ma

Background

Prolonged bradycardia leads to inadequate pumping of the heart, causing myocardial ischemia, which in turn affects cardiac function. Depending on its clinical features, improving the AV node conduction block may be an important tool to inhibit the progression of the disease. Lingbao Huxin Dan, which consists of several traditional Chinese medicines, is used for conditions similar to bradycardia and may be a potential therapeutic agent for bradycardia. In this experiment, we investigated its therapeutic significance and possible therapeutic targets for sinus bradycardia.

Methods

The bradycardia model of SD rats was prepared by using acetylcholine, propranolol, and verapamil respectively, and treated with Lingbao Huxin Dan for 3 weeks. At the end of treatment, the rats' ECG was recorded using a PV-LOOP heart rate recorder. The hearts and serum samples were collected to detect heart rate and related gene expression which were screened through network pharmacology analysis, as well as to evaluate the effect of Lingbao Huxin Dan on ion channels in the cardiac conduction system.

Results

Lingbao Huxin Dan improved the heart rate in the bradycardia model of SD rats and inhibited AV node conduction block by targeting ion channel proteins in the cardiac conduction system. The underlying molecular mechanism may be the activation of the β-adrenergic receptor-dependent cAMP/PKA signaling pathway targeted to enhance the expression of the pacing channel HCN4. In addition, Lingbao Huxin Dan prevented the deterioration of bradycardia by promoting the production of the oxygen radical scavenger SOD and inhibiting the upregulation of LDH due to cardiomyocyte damage.

Conclusions

Our study proved that Lingbao Huxin Dan alleviated bradycardia by downregulating CX45 and enhancing the expression of HCN4, ADRβ1, and TRPM7 to shorten the atrioventricular node induction period and improve sinoatrial node signaling. In addition, Lingbao Huxin Dan relieved myocardial injury induced by bradycardia by reducing the level of oxygen free radicals in the cardiac microenvironment.

{"title":"Exploring the mechanism of Lingbao Huxin Dan in the treatment of bradycardia based on atrioventricular node electrical signal conduction","authors":"Jing Zhang , Guobin Zheng , Shangjing Liu , Yaodong Miao , Shu Yang , Sheng Li , Zhihui Yang , Danping Zhuo , Rui Guo , Yang Guo , Rongjiang Shao , Yunqing Hua , Chuanrui Ma","doi":"10.1016/j.jhip.2024.12.003","DOIUrl":"10.1016/j.jhip.2024.12.003","url":null,"abstract":"<div><h3>Background</h3><div>Prolonged bradycardia leads to inadequate pumping of the heart, causing myocardial ischemia, which in turn affects cardiac function. Depending on its clinical features, improving the AV node conduction block may be an important tool to inhibit the progression of the disease. Lingbao Huxin Dan, which consists of several traditional Chinese medicines, is used for conditions similar to bradycardia and may be a potential therapeutic agent for bradycardia. In this experiment, we investigated its therapeutic significance and possible therapeutic targets for sinus bradycardia.</div></div><div><h3>Methods</h3><div>The bradycardia model of SD rats was prepared by using acetylcholine, propranolol, and verapamil respectively, and treated with Lingbao Huxin Dan for 3 weeks. At the end of treatment, the rats' ECG was recorded using a PV-LOOP heart rate recorder. The hearts and serum samples were collected to detect heart rate and related gene expression which were screened through network pharmacology analysis, as well as to evaluate the effect of Lingbao Huxin Dan on ion channels in the cardiac conduction system.</div></div><div><h3>Results</h3><div>Lingbao Huxin Dan improved the heart rate in the bradycardia model of SD rats and inhibited AV node conduction block by targeting ion channel proteins in the cardiac conduction system. The underlying molecular mechanism may be the activation of the β-adrenergic receptor-dependent cAMP/PKA signaling pathway targeted to enhance the expression of the pacing channel HCN4. In addition, Lingbao Huxin Dan prevented the deterioration of bradycardia by promoting the production of the oxygen radical scavenger SOD and inhibiting the upregulation of LDH due to cardiomyocyte damage.</div></div><div><h3>Conclusions</h3><div>Our study proved that Lingbao Huxin Dan alleviated bradycardia by downregulating CX45 and enhancing the expression of HCN4, ADRβ1, and TRPM7 to shorten the atrioventricular node induction period and improve sinoatrial node signaling. In addition, Lingbao Huxin Dan relieved myocardial injury induced by bradycardia by reducing the level of oxygen free radicals in the cardiac microenvironment.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 333-343"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network pharmacology-based study on the mechanism of Yiwei Decoction in chronic atrophic gastritis and experimental assessment

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 DOI: 10.1016/j.jhip.2024.11.004

Zepeng Zhang , Ju Liu , Yi Wang , Xiwen Li , Manman Guo , Menglei Ding , Tongtong Zhu , Lei Zhang

Objective

Yiwei Decoction (YWD) is an ancient TCM formula with historical use in treating chronic atrophic gastritis (CAG). However, its pharmacodynamic mechanisms remain poorly understood. This study aims to study the effect of YWD on CAG through network pharmacology and experimental verification.

Methods

UPLC/MS and the SwissADME database were employed to identify active compounds in YWD. YWD targets were screened by SwissTargetPrediction databases, CAG targets were screened by databases, and the two were intersected for PPI network analysis. The target prediction was performed by GO and KEGG analysis. H₂O₂-induced injury models were established in zebrafish and GES-1 cells, which were subsequently treated with varying doses of YWD. Oxidative stress indicators and apoptosis and inflammation levels in zebrafish and GES-1 cells were assessed using fluorescence microscopy, flow cytometry, and microplate reader assays. The binding capabilities of the core components and core targets were examined using molecular docking. Q-PCR and Western blot were employed to analyze the expression levels of Nrf2, Keap1, and HO-1, respectively.

Results

Forty-nine compounds were identified from YWD. Network pharmacological analysis suggested that YWD may treat CAG by modulating redox-related signaling pathways, inhibiting apoptosis, and reducing inflammation. Subsequent in vitro and in vivo experiments validated these predictions. YWD effectively mitigated H₂O₂-induced oxidative stress in zebrafish and GES-1 cells, suppressing ROS and decreasing apoptosis. YWD reduced inflammation in gastric epithelial cells. Molecular docking results suggested that methylophiopogonanone A and imperatorin may play a key role in the treatment of YWD. Mechanistically, YWD activated the Nrf2 signaling pathway, downregulated Keap1 expression, and upregulated HO-1 and Bcl2 expression.

Conclusion

YWD could improve oxidative stress indicators, inhibiting cell apoptosis, reducing inflammation, and its molecular mechanism of the CAG treatment may be through the Keap1/Nrf2/HO-1 pathways, and to promote the body's antioxidant system.

{"title":"Network pharmacology-based study on the mechanism of Yiwei Decoction in chronic atrophic gastritis and experimental assessment","authors":"Zepeng Zhang , Ju Liu , Yi Wang , Xiwen Li , Manman Guo , Menglei Ding , Tongtong Zhu , Lei Zhang","doi":"10.1016/j.jhip.2024.11.004","DOIUrl":"10.1016/j.jhip.2024.11.004","url":null,"abstract":"<div><h3>Objective</h3><div>Yiwei Decoction (YWD) is an ancient TCM formula with historical use in treating chronic atrophic gastritis (CAG). However, its pharmacodynamic mechanisms remain poorly understood. This study aims to study the effect of YWD on CAG through network pharmacology and experimental verification.</div></div><div><h3>Methods</h3><div>UPLC/MS and the SwissADME database were employed to identify active compounds in YWD. YWD targets were screened by SwissTargetPrediction databases, CAG targets were screened by databases, and the two were intersected for PPI network analysis. The target prediction was performed by GO and KEGG analysis. H<sub>2</sub>O<sub>2</sub>-induced injury models were established in zebrafish and GES-1 cells, which were subsequently treated with varying doses of YWD. Oxidative stress indicators and apoptosis and inflammation levels in zebrafish and GES-1 cells were assessed using fluorescence microscopy, flow cytometry, and microplate reader assays. The binding capabilities of the core components and core targets were examined using molecular docking. Q-PCR and Western blot were employed to analyze the expression levels of Nrf2, Keap1, and HO-1, respectively.</div></div><div><h3>Results</h3><div>Forty-nine compounds were identified from YWD. Network pharmacological analysis suggested that YWD may treat CAG by modulating redox-related signaling pathways, inhibiting apoptosis, and reducing inflammation. Subsequent <em>in vitro</em> and <em>in vivo</em> experiments validated these predictions. YWD effectively mitigated H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in zebrafish and GES-1 cells, suppressing ROS and decreasing apoptosis. YWD reduced inflammation in gastric epithelial cells. Molecular docking results suggested that methylophiopogonanone A and imperatorin may play a key role in the treatment of YWD. Mechanistically, YWD activated the Nrf2 signaling pathway, downregulated Keap1 expression, and upregulated HO-1 and Bcl2 expression.</div></div><div><h3>Conclusion</h3><div>YWD could improve oxidative stress indicators, inhibiting cell apoptosis, reducing inflammation, and its molecular mechanism of the CAG treatment may be through the Keap1/Nrf2/HO-1 pathways, and to promote the body's antioxidant system.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 4","pages":"Pages 277-290"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143174792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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