Antibiotic Susceptibility of Major Bacteria Cause Ovine Mastitis in River Nile State, Sudan

Abstract

In this study a total of 40 milk samples positive for California Mastitis Test (CMT) were collected from ewes in the three localities of River Nile State (Atbara, Barbar and El Damer). Samples were submitted for bacteriological examination. The aerobic bacteria isolated and identified from ewes’ milk samples were 19 Staphylococci  (21.0%), 9 Streptococci (9.9%), 5 Echerichia coli (5.6%), 4 Corynebacterium bovis (3.3%), 3 Enterobacter spp. (3.3%), 2 Klebsiella pneumoniae spp, (2.2%) and  2 Enterococcus faecalis (2.2%). All isolates were subjected to sensitivity tests using 10 antibiotics in use for treatment of mastitis in Sudan. 89.5% Staphylococcal isolates were sensitive to Ampicillin, 73.3% to Cephalothin, 68.4% to Ciprofloxacin and Kanamycin, 63.2% to Gentamycin, 57.9% to Chloramphenicol, 26.3% to Tetracycline, 15.8% to Procaine Penicillin and Cloxacillin and 5.3 to Erythromycin. 88.9% of sterptococcal isolates were sensetive to  Ampicillin, 66.7% to Gentamycin, 55.6% to Ciprofloxacin and Kanamycin, 44.5% to Cephalothin, 33.4% to Chloramphenicol, 22.2% to Procaine Penicillin and Cloxacillin and 11.1% to Erythromycin and all strains were resistant to Tetracycline. 80% of Echerichia coli isolates were sensitive to Ciprofloxacin, 60.0% to Ampicillin, Cephalothin, Chloramphenicol and Tetracycline, 40.0% to Gentamycin and Kanamycin, 20.0% to Erythromycin and Cloxacillin and all strains were resistant to Procaine Penicillin. All Corynebacterium bovis isolates were sensitive to Gentamycin, 66.6% to Cephalothin, 33.3% to Ampicillin, Kanamycin, Tetracycline and Cloxacillin and all strains were resistant to Chloramphenicol,  Ciprofloxacin, Erythromycin, and Procaine Penicillin. All Enterococcus faecalis isolates were sensitive to Ciprofloxacin and Gentamycin. 50.0% to Cephalothin, Chloramphenicol, Kanamycin and Tetracycline. All strains were resistant to Ampicillin, Erythromycin, Procaine Penicillin and Cloxacillin. 50.0% of Klebsiella pneumoniae isolates were sensitive to Ciprofloxacin, Gentamycin and Kanamycin. All strains were resistant to Ampicillin, Cephalothin, Chloramphenicol, Erythromycin Procaine Penicillin,  Cloxacillin and Tetracycline. All Enterobacter spp. Isolates were sensetive to Cephalothin and Chloramphenicol, 66.7% to Ciprofloxacin, Tetracycline and Gentamycin and all isolates were resstant to Ampicillin, Erythromycin, Kanamycin, Procaine Penicillin and Cloxacillin.