Detection of Molecular Differences of Klebsiella Isolated from Groundnut from Sudan and Argentina Using 16srrna and Nifh Genes Sequences Alignment

Abstract

For many years many crop inoculation programs were achieved to increase production. Some of these programs succeeded and others failed. The reasons of failure may be lack of genetic information of the inoculants used, and how the differences in the genes sequences influence nitrogen fixation rates. Thus, this study was done to explore differences in 16SrRNA and nifH genes sequences of Klebsiella isolated from groundnut from Sudan and Argentina. The bacterial strains were isolated from groundnut grown in different regions in Sudan, DNA was extracted and 16SrRNA and nifH genes were amplified and sequenced. The Argentinean isolates sequences were downloaded from Gene bank data base. The sequences of the isolates from Sudan and Argentina were aligned. All Klebsiella strains used in this study were found with similar GC contents. The results showed that 16srRNA genes of isolates from Sudan differ in 34 and 54 nucleotide positions when compared to TT001 and NTI31 isolates from Argentina, respectively. The nifH genes of the isolates from Sudan are similar and those from Argentina are also similar, however each group differ from the other in 28 positions. The genetic properties knowledge for bacteria associated with legumes helps to select the suitable isolates to use as inoculants for specific region which in turn leads to successful inoculation programs.