Miranda F. Kamal, Sohila M. Elonsy, H. Labib, Sameh E. Younis
{"title":"Simultaneous MEKC-DAD and smart spectrophotometric assays of thiocolchicoside and etoricoxib in challenging concentration ratio mixtures","authors":"Miranda F. Kamal, Sohila M. Elonsy, H. Labib, Sameh E. Younis","doi":"10.1515/revac-2022-0038","DOIUrl":null,"url":null,"abstract":"Abstract Potent muscle relaxant (thiocolchicoside, TCC) and nonsteroidal anti-inflammatory drug (etoricoxib, ETXB) fixed-dose combination is formulated at relatively high 1:15 and 1:7.5 ratios for TCC and ETXB, respectively. Since the minor component (TCC) has lower absorptivity, assay of TCC/ETXB tablets presents an analytical challenge. The current study presents two novel methods: first is a micellar electrokinetic capillary chromatography (MEKC). Background electrolyte is borate buffer (40 mM, pH 9.2) containing 30 mM sodium dodecyl sulfate and methanol (ratio 80:20%, v/v), measured at 210 nm. Second is a direct double A max spectrophotometric method; minor component, TCC, is measured directly at its distant λ max (373 nm), at zero absorption of ETXB. Then, a ten-fold dilution step is carried out to eliminate TCC spectral interference and ETXB is determined at its λ max (282 nm). Both drugs’ concentrations disclose obedient linearities at 2–100 μg·mL−1 in MEKC, versus 3–25 and 40–350 μg·mL−1 for TCC and ETXB, respectively, in spectrophotometry. All ICH validation elements have been fulfilled for the developed methods. MEKC and spectrophotometric assays achieve accuracy, precision, selectivity, and robustness to be recommended for industrial quality control routine analysis of TCC/ETXB pills formulated at cited ratios or even higher.","PeriodicalId":21090,"journal":{"name":"Reviews in Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reviews in Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1515/revac-2022-0038","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 1
Abstract
Abstract Potent muscle relaxant (thiocolchicoside, TCC) and nonsteroidal anti-inflammatory drug (etoricoxib, ETXB) fixed-dose combination is formulated at relatively high 1:15 and 1:7.5 ratios for TCC and ETXB, respectively. Since the minor component (TCC) has lower absorptivity, assay of TCC/ETXB tablets presents an analytical challenge. The current study presents two novel methods: first is a micellar electrokinetic capillary chromatography (MEKC). Background electrolyte is borate buffer (40 mM, pH 9.2) containing 30 mM sodium dodecyl sulfate and methanol (ratio 80:20%, v/v), measured at 210 nm. Second is a direct double A max spectrophotometric method; minor component, TCC, is measured directly at its distant λ max (373 nm), at zero absorption of ETXB. Then, a ten-fold dilution step is carried out to eliminate TCC spectral interference and ETXB is determined at its λ max (282 nm). Both drugs’ concentrations disclose obedient linearities at 2–100 μg·mL−1 in MEKC, versus 3–25 and 40–350 μg·mL−1 for TCC and ETXB, respectively, in spectrophotometry. All ICH validation elements have been fulfilled for the developed methods. MEKC and spectrophotometric assays achieve accuracy, precision, selectivity, and robustness to be recommended for industrial quality control routine analysis of TCC/ETXB pills formulated at cited ratios or even higher.
期刊介绍:
Reviews in Analytical Chemistry publishes authoritative reviews by leading experts in the dynamic field of chemical analysis. The subjects can encompass all branches of modern analytical chemistry such as spectroscopy, chromatography, mass spectrometry, electrochemistry and trace analysis and their applications to areas such as environmental control, pharmaceutical industry, automation and other relevant areas. Review articles bring the expert up to date in a concise manner and provide researchers an overview of new techniques and methods.