ACE I/D polymorphism is not a genetic modifier of renal features in sickle cell anemia patients

Abstract

Introduction: Sickle cell anemia (SCA) exhibits a host of complications that contribute to increased morbidity and mortality at the youngest ages. Objectives: The aim of this investigation is to look into the association between ACE I/D polymorphism and renal function in Indian patients with SCA. Patients and Methods: About 190 SCA patients confirmed by hemoglobin (Hb) electrophoresis were selected for this study. The severity of the disease was determined using anemia, clinical complications, total white blood cells count, and scores of blood transfusion. To define different renal function phases, estimated glomerular filtration rate (eGFR) was computed in adults and children using the CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) and Schwartz equations respectively. The ACE I/D polymorphism was conducted using polymerase chain reaction (PCR) and separation through agarose electrophoresis. Results: The risk of impaired renal function was not statistically distinct between ACE I/D genotypes and alleles. Further, the genotypes of ACE I/D and the risk of disease severity was not found to be associated with each other. Conclusion: This investigation found that ACE I/D is an insignificant genetic modifier of renal function or severity of disease in patients with SCA.