Essai de conservation prolongée de gamètes à basse température, sans congélation

Abstract

Trial of long-term gamete storage at low temperature without freezing. In this study we attempted long-term storage of living cells (particularly gametes) at a temperature lower than 0 OC. Contrary to other methods, avoiding cell disorganization no anti-crystallization agent was used. Preservation was obtained by lowering the freezing point of the cell medium by increasing the pressure. The cells thus treated emerged from the tests apparently intact, but the spermatozoa were not motile.