COCCINIA GRANDIS FRUIT EXTRACT GEL FOR THE TREATMENT OF MOUTH ULCER ALONG WITH ASSOCIATED WOUND AND INFLAMMATION

Abstract

Culinary utility of the Coccinia grandis (Cucurbitaceae) indicates the use of fruits in the form of different recepies. Coccinia grandis (Cucurbitaceae) is a perennial plant popularly known as kundru, Tondlee, Ivy gourd, Bimbi and Scarlet gourd etc. This herb is used in folk medicine in the form of intact fruits for healing oral ulcers. So the present work was done with an objective to develop a gel formulation from hydroalcoholic extract of coccinia grandis fruit for the treatment of ulcer and for healing of associated wound and inflammation. Molecular docking study was carried out for lupeol and taraxerone, the chemical constituents contained in the fruits. The dock score values were found to be -51.83 and -35.87 respectively for antiulcer activity. For wound healing activity the values found were -27.25 and -27.17 respectively for the two components, whereas the dock score values obtained for the anti-inflammatory activities were -58.6632 and -58. respectively for lupeol and taraxerone. The negative dock score value shows better affinity to bind receptor site. Application of hydroalcoholic extract gel of (20%w/w) for antiulcer and wound healing activities caused a significant reduction in ulcer and wound area when compared with the untreated controls and marketed formulation. Topical application of gel at 20%w/w concentration showed significant reduction in carrageenan induced rat paw edema. Studies carried out for Antiulcer, Wound Healing and Anti-Inflammatory activities showed promising results proving the standardized utilization of traditional herb having culinary applicability. Moreover the data was also supported by the results of computational studies of major constituents responsible for the said activities. The approach in the present study was fruitful to prove the prospectives of hydroalcoholic extract gel of Coccinia grandis fruit as the effective treatment for ulcer and healing associated with wound and inflammation.