Preliminary anti-microbial and phytochemical investigation of the extracts and column fractions of Alchornea Floribunda leaves

Journal of Pharmaceutical and Allied Sciences Pub Date : 2009-12-01 DOI:10.4314/JOPHAS.V4I1.48450

F. Okoye, G. Ebi

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引用次数: 6

Abstract

The leaves of Alchornea floribunda were collected, identified and reduced to coarse powder. Using various solvent treatments, the powdered drug leaf was fractionated into A1, A2, B, C and D. The fractions were subjected to anti-microbial screening and phytochemical analysis. Phytochemical analysis of the extracts showed the presence of terpenes, sterols, flavonoids, tannins, carbohydrate, glycoside, saponins and alkaloids. Only fraction A2 (the terpenoid fraction) showed promising antimicrobial activity against Pseudomonas aeruginosa (IZD 42 mm) Salmonella keitambii (IZD 34mm) and Bacillus subtilis (IZD 32mm). A2 was further separated into 14 major column chromatographic fractions. The fractions were subjected to anti-microbial and phytochemical investigation. Fraction Af7, Af9, Af12 and Af13 (all terpenoids) showed mild activity against Pseudomonas aeruginosa and Salmonella keitambii. In conclusion, the anti-microbial activity of Alchornea floribunda leaves can be attributed to the presence of the terpenoids which may interact synergistically to cause inhibition of the sensitive bacteria.

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花竹叶提取物及柱状组分的抑菌及植物化学初步研究

收集并鉴定了花竹的叶片，并将其还原为粗粉。采用不同的溶剂处理方法，将药叶粉末状分离成A1、A2、B、C和d，进行抗菌筛选和植物化学分析。植物化学分析表明，提取物中含有萜烯、甾醇、黄酮类、单宁、碳水化合物、糖苷、皂苷和生物碱。只有A2(萜类成分)对铜绿假单胞菌(IZD 42 mm)、keitambii沙门氏菌(IZD 34mm)和枯草芽孢杆菌(IZD 32mm)具有良好的抑菌活性。A2进一步分离为14个主要柱层析组分。对其进行了抗菌和植物化学研究。Af7、Af9、Af12和Af13均为萜类化合物，对铜绿假单胞菌和凯氏沙门氏菌均有较强的抗氧化活性。综上所述，藻藻叶的抗菌活性可能与萜类化合物的存在有关，萜类化合物可能协同作用对敏感菌产生抑制作用。

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Journal of Pharmaceutical and Allied Sciences

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