Development and clinical application of DNA probe specific for Peptostreptococcus micros.

S. Yasui
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引用次数: 9

Abstract

P. micros is thought to be an important pathogen in the etiology of certain inflammatory lesions, however, the role of this microorganism is uncertain due to the lack of rapid and reliable method to identify this species. The purpose of this study was to develop a DNA probe specific for the detection of P. micros in order to evaluate its prevalence in oral infectious lesions. The whole genomic DNA from P. micros was partially digested and inserted into the vector pUC 13. Four recombinant clones were selected, purified and screened against reference strains of Peptostreptococcus species to check the species specificity and then applied to clinical isolates. The sensitivity and specificity of the DNA probe for P. micros was 99.2% and 100%, respectively. P. micros could be detected in 7.2% of subgingival dental plaques from the patients with adult periodontitis and in 16.1% of the endodontic lesions with periapical pathosis. In the endodontic lesions, there was a good correlation between the clinical symptoms and the presence of P. micros. These data strongly suggested that the DNA probe can be useful in the detection of P. micros and that this microorganism is important in certain periodontal and endodontic lesions.
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微胃链球菌特异性DNA探针的研制及临床应用。
P. micros被认为是某些炎症病变病因学中的重要病原体,然而,由于缺乏快速可靠的方法来鉴定该物种,该微生物的作用尚不确定。本研究的目的是开发一种特异性的DNA探针来检测微假单胞菌,以评估其在口腔感染性病变中的患病率。将全基因组DNA部分消化后插入载体puc13中。选择4个重组克隆,对其进行纯化和对照筛选,检验菌株的特异性,并应用于临床分离。DNA探针对微孢子虫的敏感性和特异性分别为99.2%和100%。成人牙周炎患者龈下菌斑中有7.2%检出微孢子虫,根尖周病变的牙髓病变中有16.1%检出微孢子虫。在牙髓病变中,临床症状与微孢子虫的存在有很好的相关性。这些数据强烈表明,DNA探针可用于检测微假单胞菌,这种微生物在某些牙周和牙髓病变中很重要。
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