Structure Based Computational Analysis and Molecular Phylogeny of C- Phycocyanin Gene from the Selected Cyanobacteria

World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering Pub Date : 2013-09-04 DOI:10.5281/ZENODO.1088072

N. Reehana, A. P. Ahamed, D. M. Ali, A. Suresh, R. Kumar, N. Thajuddin

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引用次数: 10

Abstract

Cyanobacteria play a vital role in the production of phycobiliproteins that includes phycocyanin and phycoerythrin pigments. Phycocyanin and related phycobiliproteins have wide variety of application that is used in the food, biotechnology and cosmetic industry because of their color, fluorescent and antioxidant properties. The present study is focused to understand the pigment at molecular level in the Cyanobacteria Oscillatoria terebriformis NTRI05 and Oscillatoria foreaui NTRI06. After extraction of genomic DNA, the amplification of C-Phycocyanin gene was done with the suitable primer PCβF and PCαR and the sequencing was performed. Structural and Phylogenetic analysis was attained using the sequence to develop a molecular model. Keywords—Cyanobacteria, C-Phycocyanin gene, Phylogenetic analysis, Structural analysis.

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蓝藻C-藻蓝蛋白基因的结构计算分析及分子系统发育

蓝藻在藻胆蛋白的生产中起着至关重要的作用，藻胆蛋白包括藻蓝蛋白和藻红蛋白色素。藻蓝蛋白及其相关藻胆蛋白因其具有显色、荧光和抗氧化等特性，在食品、生物技术和化妆品等领域有着广泛的应用。本研究的重点是在分子水平上了解无色蓝藻振荡菌NTRI05和前蓝藻振荡菌NTRI06中的色素。提取基因组DNA后，用合适的引物PCβF和PCαR扩增c -藻蓝蛋白基因，并进行测序。利用该序列进行了结构和系统发育分析，建立了分子模型。关键词:蓝藻;c -藻蓝蛋白基因;系统发育分析;

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World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering

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