Phytochemical Screening and In-vitro Antibacterial Activity of Mangifera indica (Mango) Kernel on Aeromonas caviae

Abstract

OBJECTIVE: To determine the phytoconstituents of Mangifera indica kernel and its antibacterial activity against Aeromonas caviae. METHODS: Methanol extract of mango kernel was used to identify secondary metabolites of mango kernel. Aqueous and methanol extracts of mango kernel were tested in-vitro for antibacterial activity. Colony of pure culture of A. caviae was inoculated onto sterile plate of Muller-Hinton agar. Five wells (6mm in diameter) were made in circular pattern equidistance in each of the plates using a sterile cup borer. One hundred microliters of different concentrations of both aqueous and methanol extracts of mango kernel were placed into the wells using sterile pipettes and 100μl of sterile distilled water in a given well as negative control. The test was performed in triplicates (3 plates for every extract), the plates were incubated at 37oC for 24 hours. RESULTS: Phytochemical screening revealed the presence of Carbohydrates, Triterpenes, Unsaturated sterols, Cardiac glycosides, Saponins, Tannins and Flavonoids. Methanol extract used at different concentrations showed varying degrees of inhibition against A. caviae (ranging from 16 ± 2.41 mm to 24 ± 0.58 mm) except that the aqueous extract was found to be slightly active against the organism at lower concentrations with zones of inhibition ranging from 8 ± 1.22 mm to 11 ± 1.23 mm with measurable zones of inhibition at higher concentrations. CONCLUSION: Methanol extract of mango kernel at different concentrations inhibited the growth of A. caviae.